The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase.

Journal Article (Journal Article)

The Arabidopsis SLY1 (SLEEPY1) gene positively regulates gibberellin (GA) signaling. Positional cloning of SLY1 revealed that it encodes a putative F-box protein. This result suggests that SLY1 is the F-box subunit of an SCF E3 ubiquitin ligase that regulates GA responses. The DELLA domain protein RGA (repressor of ga1-3) is a repressor of GA response that appears to undergo GA-stimulated protein degradation. RGA is a potential substrate of SLY1, because sly1 mutations cause a significant increase in RGA protein accumulation even after GA treatment. This result suggests SCF(SLY1)-targeted degradation of RGA through the 26S proteasome pathway. Further support for this model is provided by the observation that an rga null allele partially suppresses the sly1-10 mutant phenotype. The predicted SLY1 amino acid sequence is highly conserved among plants, indicating a key role in GA response.

Full Text

Duke Authors

Cited Authors

  • McGinnis, KM; Thomas, SG; Soule, JD; Strader, LC; Zale, JM; Sun, T-P; Steber, CM

Published Date

  • May 2003

Published In

Volume / Issue

  • 15 / 5

Start / End Page

  • 1120 - 1130

PubMed ID

  • 12724538

Pubmed Central ID

  • PMC153720

Electronic International Standard Serial Number (EISSN)

  • 1532-298X

International Standard Serial Number (ISSN)

  • 1040-4651

Digital Object Identifier (DOI)

  • 10.1105/tpc.010827


  • eng