Ethanol inhibition of AMPA and kainate receptor-mediated depolarizations of hippocampal area CA1.

Published

Journal Article

Longitudinal hippocampal slices were prepared from adult female rats. The excitatory amino acids, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) and kainic acid, were applied to area CA1, and the resulting depolarizations were measured using the grease-gap electrophysiological technique. Agonist dose-response curves were generated in the presence and absence of various concentrations of ethanol. Ethanol (25-200 mM) significantly attenuated the depolarizations that were produced by each agonist. In addition, we found that ethanol potently antagonized kainate-induced depolarizations across the agonist concentration-response curve, whereas it significantly suppressed only AMPA responses that were induced with moderate-to-high agonist concentrations. These results indicate that ethanol has potent antagonist actions against non-N-methyl-D-aspartate (NMDA) excitatory amino acid-induced neuronal depolarizations in hippocampal area CA1. Moreover, the relative potency of ethanol depends on the specific excitatory agonist tested and the concentration of that agonist. This suggests that, in addition to the known effects of ethanol on NMDA receptor-mediated activity, it may also potently attenuate ongoing "fast" glutamatergic synaptic activity in the hippocampus.

Full Text

Duke Authors

Cited Authors

  • Martin, D; Tayyeb, MI; Swartzwelder, HS

Published Date

  • October 1995

Published In

Volume / Issue

  • 19 / 5

Start / End Page

  • 1312 - 1316

PubMed ID

  • 8561307

Pubmed Central ID

  • 8561307

International Standard Serial Number (ISSN)

  • 0145-6008

Digital Object Identifier (DOI)

  • 10.1111/j.1530-0277.1995.tb01617.x

Language

  • eng

Conference Location

  • England