Vacuolar and plasma membrane stripping and autophagic elimination of Toxoplasma gondii in primed effector macrophages

Published

Journal Article

Apicomplexan protozoan pathogens avoid destruction and establish a replicative niche within host cells by forming a nonfusogenic parasitophorous vacuole (PV). Here we present evidence for lysosome-mediated degradation of Toxoplasma gondii after invasion of macrophages activated in vivo. Pathogen elimination was dependent on the interferon γ inducible-p47 GTPase, IGTP, required PI3K activity, and was preceded by PV membrane indentation, vesiculation, disruption, and, surprisingly, stripping of the parasite plasma membrane. Denuded parasites were enveloped in autophagosome-like vacuoles, which ultimately fused with lysosomes. These observations outline a series of mechanisms used by effector cells to redirect the fate of a classically nonfusogenic intracellular pathogen toward a path of immune elimination. JEM © The Rockefeller University Press.

Full Text

Duke Authors

Cited Authors

  • Ling, YM; Shaw, MH; Ayala, C; Coppens, I; Taylor, GA; Ferguson, DJP; Yap, GS

Published Date

  • September 4, 2006

Published In

Volume / Issue

  • 203 / 9

Start / End Page

  • 2063 - 2071

Electronic International Standard Serial Number (EISSN)

  • 0022-1007

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.20061318

Citation Source

  • Scopus