Bone marrow dendritic cell-mediated regulation of TLR and B cell receptor signaling in B cells.

Published

Journal Article

Dendritic cells (DCs) play an essential role in regulation of immune responses. In the periphery, Ag presentation by DCs is critical for adaptive responses; for this reason, DCs are often targets of adjuvants that enhance vaccine responses. Activated mature DCs enhance B cell activation and differentiation by providing cytokines like BAFF and a proliferation-inducing ligand. However, the role of immature DCs in B cell tolerance is not well studied. Recently, mouse immature bone marrow-derived DCs (iBMDCs) have been shown to suppress anti-IgM-induced B cell activation. In this study, we tested the ability of mouse DCs to modulate B cell functions during TLR activation. We found that iBMDCs potently suppressed proliferation and differentiation of various B cell subsets on TLR stimulation. However, iBMDCs did not affect CD40-mediated B cell activation. Optimal suppression of B cell activation by iBMDCs required cell contact via the CD22 receptor on B cells. The B cell suppression was a property of iBMDCs or DCs resident in the bone marrow (BM), but not mature BM-derived DCs or DCs resident in the spleen. Presence of iBMDCs also enhanced the Ag-induced apoptotic response of BM B cells, suggesting that the suppressive effects of iBMDCs may have a role in B cell tolerance.

Full Text

Duke Authors

Cited Authors

  • Sindhava, VJ; Tuna, H; Gachuki, BW; DiLillo, DJ; Avdiushko, MG; Onami, TM; Tedder, TF; Cohen, DA; Bondada, S

Published Date

  • October 1, 2012

Published In

Volume / Issue

  • 189 / 7

Start / End Page

  • 3355 - 3367

PubMed ID

  • 22942427

Pubmed Central ID

  • 22942427

Electronic International Standard Serial Number (EISSN)

  • 1550-6606

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.1101352

Language

  • eng

Conference Location

  • United States