Lymphocyte migration and humoral immune responses in L-selectin deficient mice
L-selectin is involved in attachment of lymphocytes to high endochelial venules (HEV) of peripheral lymph nodes (PLN) and mediates the rolling of leukocytes upon inflamed endothelium. This study, using Lselectin deficient mice, investigated the effects of L-selectin deficiency on HEV function, the distribution of lymphocyte subpopulations and the generation of immune responses. The HEV of L-selectin deficient mice were phenotypically and functionally comparable to that of wild-type mice. In blood, L-selectin deficient mice had a 2- to 3-fold increase in the number of monocytes and a 2-fold increase in the number of CD4+ T cells with a 33% decrease in B cells. CD4+ T cells expressing a memory phenotype (CD18hi CD441) increased in both the spleen and blood of L-selectin deficient mice by 60% and 300%, respectively. L-selectin deficient mice showed elevated humoral immune responses following i.p. inoculation with either DNP-Ficoll or DNP-KLH. In contrast, the hapten-specific IgM and IgG responses were decreased by 40% and 92%, respectively, in Lselectin deficient mice following footpad immunization with DNP-KLH. Following a secondary inoculation of DNP-KLH, the L-selectin deficient mice mounted an increased humoral response regardless of the route of inoculation. Thus, the lack of L-selectin results in a marked redistribution of lymphocyte subsets including the accumulation of memory cells within lymphoid tissues. While L-selectin is not required for the generation of humoral immune responses, it does facilitate a more rapid primary immune response to peripheral antigenic challenge.
Steeber, DA; Green, NE; Sato, S; Tedder, TP
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