A copper-sensing transcription factor regulates iron uptake genes in Schizosaccharomyces pombe.

Published

Journal Article

Copper and iron serve essential functions as catalytic co-factors in a wide variety of critical cellular enzymes. Studies in yeast have demonstrated an absolute dependence upon copper acquisition for proper assembly and function of the iron transport machinery. We have cloned genes for a high affinity copper transporter (Ctr4) and copper-sensing transcription factor (Cuf1) from Schizosaccharomyces pombe. Interestingly, the primary structure of Ctr4 and a putative human high affinity copper transport protein, hCtr1, suggests that they are derived from a fusion of the functionally redundant but structurally distinct Ctr1 and Ctr3 copper transporters from Saccharomyces cerevisiae. Furthermore, although Cuf1 activates ctr4(+) gene expression under copper starvation conditions, under these same conditions Cuf1 directly represses expression of genes encoding components of the iron transport machinery. These studies have identified an evolutionary step in which copper transport modules have been fused, and describe a mechanism by which a copper-sensing factor directly represses expression of the iron uptake genes under conditions in which the essential copper co-factor is scarce.

Full Text

Duke Authors

Cited Authors

  • Labbé, S; Peña, MM; Fernandes, AR; Thiele, DJ

Published Date

  • December 17, 1999

Published In

Volume / Issue

  • 274 / 51

Start / End Page

  • 36252 - 36260

PubMed ID

  • 10593913

Pubmed Central ID

  • 10593913

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.274.51.36252

Language

  • eng

Conference Location

  • United States