Development of a serological test for Haemophilus ducreyi for seroprevalence studies

Journal Article

We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specificity and to establish cutoff values. On the basis of these data, rpEIA was found to be both sensitive and specific when used to test a variety of serum specimens from patients with genital ulcers and urethritis and from healthy blood donors.

Duke Authors

Cited Authors

  • Elkins, C; Yi, K; Olsen, B; Thomas, C; Thomas, K; Morse, S

Published Date

  • 2000

Published In

  • Journal of Clinical Microbiology

Volume / Issue

  • 38 / 4

Start / End Page

  • 1520 - 1526

PubMed ID

  • 10747137