Development of a serological test for Haemophilus ducreyi for seroprevalence studies.
Journal Article (Journal Article)
We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specificity and to establish cutoff values. On the basis of these data, rpEIA was found to be both sensitive and specific when used to test a variety of serum specimens from patients with genital ulcers and urethritis and from healthy blood donors.
Full Text
Duke Authors
Cited Authors
- Elkins, C; Yi, K; Olsen, B; Thomas, C; Thomas, K; Morse, S
Published Date
- April 2000
Published In
Volume / Issue
- 38 / 4
Start / End Page
- 1520 - 1526
PubMed ID
- 10747137
Pubmed Central ID
- PMC86480
International Standard Serial Number (ISSN)
- 0095-1137
Digital Object Identifier (DOI)
- 10.1128/JCM.38.4.1520-1526.2000
Language
- eng
Conference Location
- United States