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Continuous-flow determination of dialyzable calcium in serum.

Publication ,  Journal Article
Toffaletti, J; Savory, J; Gitelman, HJ
Published in: Clin Chem
July 1977

We describe a procedure for measuring dialyzable calcium in 60 sera per hour with use of AutoAnalyzer (Technicon) modules. The serum is buffered, then dialyzed in a regular 6-inch (15-cm) dialysis block. A constant proportion of the dialyzable calcium is fluorometrically measured. Analysis of samples containing protein, prepared by an equilibrium dialysis technique, shows that protein-bound calcium is not detected. The method requires less than 0.4 ml of serum, and is accurate, precise, and durable. No unusual care or special techniques are required during sample collection or analysis.

Duke Scholars

Published In

Clin Chem

ISSN

0009-9147

Publication Date

July 1977

Volume

23

Issue

7

Start / End Page

1258 / 1263

Location

England

Related Subject Headings

  • Tromethamine
  • Reference Values
  • Permeability
  • Methods
  • Hydrogen-Ion Concentration
  • Humans
  • General Clinical Medicine
  • Dialysis
  • Calcium
  • Adult
 

Citation

APA
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ICMJE
MLA
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Toffaletti, J., Savory, J., & Gitelman, H. J. (1977). Continuous-flow determination of dialyzable calcium in serum. Clin Chem, 23(7), 1258–1263.
Toffaletti, J., J. Savory, and H. J. Gitelman. “Continuous-flow determination of dialyzable calcium in serum.Clin Chem 23, no. 7 (July 1977): 1258–63.
Toffaletti J, Savory J, Gitelman HJ. Continuous-flow determination of dialyzable calcium in serum. Clin Chem. 1977 Jul;23(7):1258–63.
Toffaletti, J., et al. “Continuous-flow determination of dialyzable calcium in serum.Clin Chem, vol. 23, no. 7, July 1977, pp. 1258–63.
Toffaletti J, Savory J, Gitelman HJ. Continuous-flow determination of dialyzable calcium in serum. Clin Chem. 1977 Jul;23(7):1258–1263.

Published In

Clin Chem

ISSN

0009-9147

Publication Date

July 1977

Volume

23

Issue

7

Start / End Page

1258 / 1263

Location

England

Related Subject Headings

  • Tromethamine
  • Reference Values
  • Permeability
  • Methods
  • Hydrogen-Ion Concentration
  • Humans
  • General Clinical Medicine
  • Dialysis
  • Calcium
  • Adult