Separation and quantitation of serum constituents associated with calcium by gel filtration.

Published

Journal Article

Gel filtration of serum by use of polyacrylamide beads (Biogel P-2) separates total colcium into four distinct peaks: an initial peak, corresponding to protein-bound calcium; a second peak containing the calcium complexes of citrate, phosphate, lactate, and sulfate; a third peak containing calcium bicarbonate; and a fourth peak or trough corresponding to the serum ionic calcium. An eluent containing (per liter) 140 mmol of sodium, 1.1o mmol of calcium, 0.50 mmol of magnesium, and 10 mmol of 2-([tris(hydroxymethyl)methyl]amino)ethanesulfonic acid(pH 7.40 at 37 degrees C) provides physiological conditions that determine the equilibria between these calcium components. Association constants determined under these conditions permit calculation of the expected concentration of the calcium complexes in each tube of eluent, and these concentrations closely correspond to the amount of bound calcium measured experimentally. The mean distribution of calcium in healthy individuals, as determined by this method, is (per liter): calcium protein, 1.00 mmol; calcium complexes, 0.31 mmol; and ionic calcium, 1.07 mmol.

Full Text

Duke Authors

Cited Authors

  • Toffaletti, J; Gitelman, HJ; Savory, J

Published Date

  • December 1, 1976

Published In

Volume / Issue

  • 22 / 12

Start / End Page

  • 1968 - 1972

PubMed ID

  • 1000793

Pubmed Central ID

  • 1000793

International Standard Serial Number (ISSN)

  • 0009-9147

Language

  • eng

Conference Location

  • England