Skip to main content

Mitochondrial C-tract alteration in premalignant lesions of the head and neck: a marker for progression and clonal proliferation.

Publication ,  Journal Article
Ha, PK; Tong, BC; Westra, WH; Sanchez-Cespedes, M; Parrella, P; Zahurak, M; Sidransky, D; Califano, JA
Published in: Clin Cancer Res
July 2002

PURPOSE: Although mitochondrial DNA mutations have been described recently in many different tumor types, the nature and timing of such alterations remain unclear. In an effort to further examine the role of mitochondrial DNA mutations in carcinogenesis, we examined 137 premalignant lesions of the head and neck from 93 patients for DNA alterations in the poly-cytosine tract (C-tract) of the displacement loop, discovered recently to be a hot spot of mitochondrial DNA alteration. EXPERIMENTAL DESING: All premalignant lesions were tested using a length-based PCR assay, which amplified the C-tract region of mitochondrial DNA. Somatic microsatellites at six loci were also tested on a subset of patients with metachronous or synchronous lesions found to possess a mitochondrial C-tract alteration. RESULTS: Thirty-four of 93 (37%) patients harbored lesions that displayed a C-tract alteration. There was a clear increase in incidence from histologically benign hyperplasia (22%) to squamous carcinoma in situ (62%: P < 0.01). We also tested synchronous dysplastic lesions, metachronous dysplastic lesions, and normal epithelium adjacent to dysplastic epithelium with this assay. In most cases, the mitochondrial C-tract status identified a clonal relationship between these lesions. Genomic microsatellites also confirmed that a clonal relationship was present in many of these cases. CONCLUSIONS: Mitochondrial DNA alterations in the head and neck occur in the earliest premalignant lesions and demonstrate a rising incidence that parallels histological severity. These alterations are valuable as additional markers of histopathological progression.

Duke Scholars

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

July 2002

Volume

8

Issue

7

Start / End Page

2260 / 2265

Location

United States

Related Subject Headings

  • Precancerous Conditions
  • Polymerase Chain Reaction
  • Poly C
  • Oncology & Carcinogenesis
  • Mutation
  • Microsatellite Repeats
  • Humans
  • Head and Neck Neoplasms
  • DNA, Neoplasm
  • DNA, Mitochondrial
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ha, P. K., Tong, B. C., Westra, W. H., Sanchez-Cespedes, M., Parrella, P., Zahurak, M., … Califano, J. A. (2002). Mitochondrial C-tract alteration in premalignant lesions of the head and neck: a marker for progression and clonal proliferation. Clin Cancer Res, 8(7), 2260–2265.
Ha, Patrick K., Betty C. Tong, William H. Westra, Montserrat Sanchez-Cespedes, Paola Parrella, Marianna Zahurak, David Sidransky, and Joseph A. Califano. “Mitochondrial C-tract alteration in premalignant lesions of the head and neck: a marker for progression and clonal proliferation.Clin Cancer Res 8, no. 7 (July 2002): 2260–65.
Ha PK, Tong BC, Westra WH, Sanchez-Cespedes M, Parrella P, Zahurak M, et al. Mitochondrial C-tract alteration in premalignant lesions of the head and neck: a marker for progression and clonal proliferation. Clin Cancer Res. 2002 Jul;8(7):2260–5.
Ha, Patrick K., et al. “Mitochondrial C-tract alteration in premalignant lesions of the head and neck: a marker for progression and clonal proliferation.Clin Cancer Res, vol. 8, no. 7, July 2002, pp. 2260–65.
Ha PK, Tong BC, Westra WH, Sanchez-Cespedes M, Parrella P, Zahurak M, Sidransky D, Califano JA. Mitochondrial C-tract alteration in premalignant lesions of the head and neck: a marker for progression and clonal proliferation. Clin Cancer Res. 2002 Jul;8(7):2260–2265.

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

July 2002

Volume

8

Issue

7

Start / End Page

2260 / 2265

Location

United States

Related Subject Headings

  • Precancerous Conditions
  • Polymerase Chain Reaction
  • Poly C
  • Oncology & Carcinogenesis
  • Mutation
  • Microsatellite Repeats
  • Humans
  • Head and Neck Neoplasms
  • DNA, Neoplasm
  • DNA, Mitochondrial