A comparison of biological and calorimetric analyses of multivalent glycodendrimer ligands for concanavalin A

Published

Journal Article

The cluster glycoside effect - the observation that multivalent glycosides bind to their polyvalent protein receptors with apparent affinities greater than those that can be rationalized solely on the basis of valency - is by now a well established phenomenon. As part of a continuing effort to provide a molecular basis for the cluster glycoside effect, we report here the synthesis of two series of mannosylated dendritic ligands and their performance in a range of competitive and non-competitive binding assays, including hemeagglutination inhibition (HIA), enzyme-linked lectin assays (ELLA) and isothermal titration microcalorimetry (ITC). The first series of ligands contained a semi-rigid glycylglycine spacer and showed no significant performance enhancement in any binding studies. The second series of ligands contained a flexible tetraethylene glycol spacer; these ligands showed marked enhancements at tetravalent and hexavalent levels in both HIA (IC50=3 and<0.8 μM, respectively) and ITC (K(A)=6.2x104 and 1.5x106 M-1, respectively) studies. In all cases, the thermodynamic parameters of association are consistent with non-specific aggregation rather than enhanced lectin-ligand affinity. This conclusion is reinforced by the lack of enhancements in ligand activity observed in ELLA studies. Copyright (C) 2000 Elsevier Science Ltd.

Full Text

Duke Authors

Cited Authors

  • Corbell, JB; Lundquist, JJ; Toone, EJ

Published Date

  • January 28, 2000

Published In

Volume / Issue

  • 11 / 1

Start / End Page

  • 95 - 111

International Standard Serial Number (ISSN)

  • 0957-4166

Digital Object Identifier (DOI)

  • 10.1016/S0957-4166(99)00589-3

Citation Source

  • Scopus