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Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation.

Publication ,  Journal Article
Wallace, CS; Truskey, GA
Published in: American journal of physiology. Heart and circulatory physiology
August 2010

We used a direct-contact endothelial cell-smooth muscle cell (EC-SMC) co-culture to examine whether quiescent SMCs regulate the EC inflammatory response to tumor necrosis factor (TNF)-alpha. ECs were cultured under static and physiological flow conditions. Compared with TNF-alpha-treated ECs in monoculture, TNF-alpha-treated ECs in co-culture had less NF-kappaB nuclear translocation; less intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin surface protein expression; no change in TNF receptor expression, but greater Kruppel-like factor 2 (KLF2) gene expression. After flow preconditioning for 24 h at 15 dyne/cm(2), and exposure of ECs to flow and TNF-alpha for 4.5 h, ECs in co-culture had less ICAM-1, VCAM-1, and E-selectin surface protein expression. Exposure to flow greatly increased KLF2 gene expression levels in both EC cultures; as a result, ECs in co-culture and monoculture had similar levels of post-flow KLF2 gene expression. The reduced levels of TNF-alpha-induced adhesion molecule expression in co-culture required the presence of quiescent SMCs; adhesion to decellularized extracellular matrix (ECM) or co-culture with fibroblasts produced only a modest reduction in EC adhesion molecule expression. Furthermore, co-culture of quiescent SMCs and ECs on the opposite side of a 10-microm-thick porous membrane did not alter the TNF-alpha-mediated ICAM-1 surface protein expression. Although the ECM produced by SMCs plays some role in reducing TNF-alpha-mediated inflammation, these results suggest that the direct contact between ECs and quiescent SMCs is required to inhibit TNF-alpha-mediated activation.

Duke Scholars

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

August 2010

Volume

299

Issue

2

Start / End Page

H338 / H346

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Tumor Necrosis Factor-alpha
  • Time Factors
  • Stress, Mechanical
  • Recombinant Proteins
  • Receptors, Tumor Necrosis Factor
  • Pulsatile Flow
  • NF-kappa B
  • Myocytes, Smooth Muscle
  • Muscle, Smooth, Vascular
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wallace, C. S., & Truskey, G. A. (2010). Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation. American Journal of Physiology. Heart and Circulatory Physiology, 299(2), H338–H346. https://doi.org/10.1152/ajpheart.01029.2009
Wallace, Charles S., and George A. Truskey. “Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation.American Journal of Physiology. Heart and Circulatory Physiology 299, no. 2 (August 2010): H338–46. https://doi.org/10.1152/ajpheart.01029.2009.
Wallace CS, Truskey GA. Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation. American journal of physiology Heart and circulatory physiology. 2010 Aug;299(2):H338–46.
Wallace, Charles S., and George A. Truskey. “Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation.American Journal of Physiology. Heart and Circulatory Physiology, vol. 299, no. 2, Aug. 2010, pp. H338–46. Epmc, doi:10.1152/ajpheart.01029.2009.
Wallace CS, Truskey GA. Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation. American journal of physiology Heart and circulatory physiology. 2010 Aug;299(2):H338–H346.

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

August 2010

Volume

299

Issue

2

Start / End Page

H338 / H346

Related Subject Headings

  • Vascular Cell Adhesion Molecule-1
  • Tumor Necrosis Factor-alpha
  • Time Factors
  • Stress, Mechanical
  • Recombinant Proteins
  • Receptors, Tumor Necrosis Factor
  • Pulsatile Flow
  • NF-kappa B
  • Myocytes, Smooth Muscle
  • Muscle, Smooth, Vascular