Combining topographical and genetic cues to promote neuronal fate specification in stem cells.

Published

Journal Article (Review)

There is little remedy for the devastating effects resulting from neuronal loss caused by neural injury or neurodegenerative disease. Reconstruction of damaged neural circuitry with stem cell-derived neurons is a promising approach to repair these defects, but controlling differentiation and guiding synaptic integration with existing neurons remain significant unmet challenges. Biomaterial surfaces can present nanoscale topographical cues that influence neuronal differentiation and process outgrowth. By combining these scaffolds with additional molecular biology strategies, synergistic control over cell fate can be achieved. Here, we review recent progress in promoting neuronal fate using techniques at the interface of biomaterial science and genetic engineering. New data demonstrates that combining nanofiber topography with an induced genetic program enhances neuritogenesis in a synergistic fashion. We propose combining patterned biomaterial surface cues with prescribed genetic programs to achieve neuronal cell fates with the desired sublineage specification, neurochemical profile, targeted integration, and electrophysiological properties.

Full Text

Duke Authors

Cited Authors

  • Purcell, EK; Naim, Y; Yang, A; Leach, MK; Velkey, JM; Duncan, RK; Corey, JM

Published Date

  • November 12, 2012

Published In

Volume / Issue

  • 13 / 11

Start / End Page

  • 3427 - 3438

PubMed ID

  • 23098293

Pubmed Central ID

  • 23098293

Electronic International Standard Serial Number (EISSN)

  • 1526-4602

Digital Object Identifier (DOI)

  • 10.1021/bm301220k

Language

  • eng

Conference Location

  • United States