Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors.

Journal Article (Journal Article)

Embryonic stem (ES) cells and their derivatives are an important resource for developing novel cellular therapies for disease. Controlling proliferation and lineage selection, however, are essential to circumvent the possibility of tumor formation and facilitate the safe translation of ES-based therapies to man. Expression of appropriate transcription factors is one approach to direct the differentiation of ES cells towards a specific lineage and stop proliferation. Neural differentiation can be initiated in ES cells by expression of Neurogenin1 (Ngn1). In this study we investigate the effects of controlled Ngn1 expression on mouse ES (mES) cell differentiation in vitro and following grafting into the rat spinal cord. In vitro, Ngn1 expression in mES cells leads to rapid and specific neural differentiation, and a concurrent decrease in proliferation. Similarly transplantation of Ngn1-expressing mES cells into the spinal cord lead to in situ differentiation and spinal precursor formation. These data demonstrate that Ngn1 expression in mES cells is sufficient to promote neural differentiation and inhibit proliferation, thus establishing an approach to safely graft ES cells into the spinal cord.

Full Text

Duke Authors

Cited Authors

  • Lunn, JS; Pacut, C; Stern, E; Sakowski, SA; Velkey, JM; O'Shea, S; Feldman, EL

Published Date

  • April 2012

Published In

Volume / Issue

  • 46 / 1

Start / End Page

  • 59 - 68

PubMed ID

  • 22245661

Pubmed Central ID

  • PMC3299886

Electronic International Standard Serial Number (EISSN)

  • 1095-953X

Digital Object Identifier (DOI)

  • 10.1016/j.nbd.2011.12.044


  • eng

Conference Location

  • United States