Skip to main content
Journal cover image

Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.

Publication ,  Journal Article
Velkey, JM; O'Shea, KS
Published in: Genesis
September 2003

We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice. Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express small stem-loop RNA transcripts corresponding to Oct4 mRNA. Cells transfected with Oct4 RNAi constructs demonstrated reduced levels of Oct4 mRNA and exhibited characteristics of trophectodermal differentiation. These findings support the critical role of Oct4 in regulating stem cell identity and suggest that future experiments using RNAi in ES cells can elucidate the roles of other genes affecting lineage specification during differentiation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genesis

DOI

ISSN

1526-954X

Publication Date

September 2003

Volume

37

Issue

1

Start / End Page

18 / 24

Location

United States

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Time Factors
  • Stem Cells
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA Polymerase III
  • RNA Interference
  • Promoter Regions, Genetic
  • Plasmids
  • Oligonucleotides, Antisense
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Velkey, J. M., & O’Shea, K. S. (2003). Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells. Genesis, 37(1), 18–24. https://doi.org/10.1002/gene.10218
Velkey, J Matthew, and K Sue O’Shea. “Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.Genesis 37, no. 1 (September 2003): 18–24. https://doi.org/10.1002/gene.10218.
Velkey, J. Matthew, and K. Sue O’Shea. “Oct4 RNA interference induces trophectoderm differentiation in mouse embryonic stem cells.Genesis, vol. 37, no. 1, Sept. 2003, pp. 18–24. Pubmed, doi:10.1002/gene.10218.
Journal cover image

Published In

Genesis

DOI

ISSN

1526-954X

Publication Date

September 2003

Volume

37

Issue

1

Start / End Page

18 / 24

Location

United States

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Time Factors
  • Stem Cells
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA Polymerase III
  • RNA Interference
  • Promoter Regions, Genetic
  • Plasmids
  • Oligonucleotides, Antisense