Genetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolina.

Journal Article

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.

Full Text

Duke Authors

Cited Authors

  • Ceresini, PC; Shew, HD; Vilgalys, RJ; Rosewich, UL; Cubeta, MA

Published Date

  • May 2002

Published In

Volume / Issue

  • 94 / 3

Start / End Page

  • 450 - 460

PubMed ID

  • 21156516

International Standard Serial Number (ISSN)

  • 0027-5514

Language

  • eng

Conference Location

  • England