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p53-dependent control of transactivation of the Pen2 promoter by presenilins.

Publication ,  Journal Article
Dunys, J; Sevalle, J; Giaime, E; Pardossi-Piquard, R; Vitek, MP; Renbaum, P; Levy-Lahad, E; Zhang, Y-W; Xu, H; Checler, F; da Costa, CA
Published in: J Cell Sci
November 1, 2009

The senile plaques found in the brains of patients with Alzheimer's disease are mainly due to the accumulation of amyloid beta-peptides (A beta) that are liberated by gamma-secretase, a high molecular weight complex including presenilins, PEN-2, APH-1 and nicastrin. The depletion of each of these proteins disrupts the complex assembly into a functional protease. Here, we describe another level of regulation of this multimeric protease. The depletion of both presenilins drastically reduces Pen2 mRNA levels and its promoter transactivation. Furthermore, overexpression of presenilin-1 lowers Pen2 promoter transactivation, a phenotype abolished by a double mutation known to prevent presenilin-dependent gamma-secretase activity. PEN-2 expression is decreased by depletion of beta-amyloid precursor protein (APP) and increased by the APP intracellular domain (AICD). We show that AICD and APP complement for Pen2 mRNA levels in APP/APLP1-2 knockout fibroblasts. Interestingly, overexpression of presenilin-2 greatly increases Pen2 promoter transactivation. The opposite effect triggered by both presenilins was reminiscent of our previous study, which showed that these two proteins elicit antagonistic effects on p53. Therefore, we examined the contribution of p53 on Pen2 transcription. Pen2 promoter transactivation, and Pen2 mRNA and protein levels were drastically reduced in p53(-/-) fibroblasts. Furthermore, PEN-2 expression could be rescued by p53 complementation in p53- and APP-deficient cells. Interestingly, PEN-2 expression was also reduced in p53-deficient mouse brain. Overall, our study describes a p53-dependent regulation of PEN-2 expression by other members of the gamma-secretase complex, namely presenilins.

Duke Scholars

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Published In

J Cell Sci

DOI

EISSN

1477-9137

Publication Date

November 1, 2009

Volume

122

Issue

Pt 21

Start / End Page

4003 / 4008

Location

England

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcriptional Activation
  • Promoter Regions, Genetic
  • Presenilin-2
  • Presenilin-1
  • Mice, Knockout
  • Mice
  • Membrane Proteins
  • Humans
  • Fibroblasts
 

Citation

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Dunys, J., Sevalle, J., Giaime, E., Pardossi-Piquard, R., Vitek, M. P., Renbaum, P., … da Costa, C. A. (2009). p53-dependent control of transactivation of the Pen2 promoter by presenilins. J Cell Sci, 122(Pt 21), 4003–4008. https://doi.org/10.1242/jcs.051169
Dunys, Julie, Jean Sevalle, Emilie Giaime, Raphaëlle Pardossi-Piquard, Michael P. Vitek, Paul Renbaum, Ephrat Levy-Lahad, et al. “p53-dependent control of transactivation of the Pen2 promoter by presenilins.J Cell Sci 122, no. Pt 21 (November 1, 2009): 4003–8. https://doi.org/10.1242/jcs.051169.
Dunys J, Sevalle J, Giaime E, Pardossi-Piquard R, Vitek MP, Renbaum P, et al. p53-dependent control of transactivation of the Pen2 promoter by presenilins. J Cell Sci. 2009 Nov 1;122(Pt 21):4003–8.
Dunys, Julie, et al. “p53-dependent control of transactivation of the Pen2 promoter by presenilins.J Cell Sci, vol. 122, no. Pt 21, Nov. 2009, pp. 4003–08. Pubmed, doi:10.1242/jcs.051169.
Dunys J, Sevalle J, Giaime E, Pardossi-Piquard R, Vitek MP, Renbaum P, Levy-Lahad E, Zhang Y-W, Xu H, Checler F, da Costa CA. p53-dependent control of transactivation of the Pen2 promoter by presenilins. J Cell Sci. 2009 Nov 1;122(Pt 21):4003–4008.
Journal cover image

Published In

J Cell Sci

DOI

EISSN

1477-9137

Publication Date

November 1, 2009

Volume

122

Issue

Pt 21

Start / End Page

4003 / 4008

Location

England

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Transcriptional Activation
  • Promoter Regions, Genetic
  • Presenilin-2
  • Presenilin-1
  • Mice, Knockout
  • Mice
  • Membrane Proteins
  • Humans
  • Fibroblasts