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Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1.

Publication ,  Journal Article
Mitsuda, N; Ohkubo, N; Tamatani, M; Lee, YD; Taniguchi, M; Namikawa, K; Kiyama, H; Yamaguchi, A; Sato, N; Sakata, K; Ogihara, T; Vitek, MP; Tohyama, M
Published in: J Biol Chem
March 30, 2001

Upon binding to the cAMP-response element of a gene's promoter, the transcription factor known as cAMP-response element-binding protein (CREB) facilitates transcription of many different neuronal genes including those involved with synaptic function. Based on our previous reports of gene structure (GenBank accession number AF029701 ), we now demonstrate that activated CREB binds to the proximal promoter of the human presenilin-1 (PS-1) gene to activate PS-1 transcription in rat and in human neuronal cells. Specific stimulation of the N-methyl-d-aspartate subtype of neuronal glutamate receptors activates CREB and results in increased PS-1 expression. Similarly, treatment with brain-derived neurotrophic factor activates CREB and increases PS-1 expression in a dose-dependent fashion. By using adenovirus vectors expressing dominant negative forms of CREB, we were able to show that induction of PS-1 expression requires the activation of CREB. Conversely, constitutive expression of mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) results in activation of CREB and increased PS-1 expression that can be blocked by the addition of selective MEK inhibitors. Our findings suggest a hypothesis where stimulation of N-methyl-d-aspartate receptors signals CREB activation to enhance PS-1 gene product expression that contributes to normal neuronal functions.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 30, 2001

Volume

276

Issue

13

Start / End Page

9688 / 9698

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Time Factors
  • Signal Transduction
  • Sequence Analysis, DNA
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Glutamate
  • Rats
  • RNA, Messenger
 

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Mitsuda, N., Ohkubo, N., Tamatani, M., Lee, Y. D., Taniguchi, M., Namikawa, K., … Tohyama, M. (2001). Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1. J Biol Chem, 276(13), 9688–9698. https://doi.org/10.1074/jbc.M006153200
Mitsuda, N., N. Ohkubo, M. Tamatani, Y. D. Lee, M. Taniguchi, K. Namikawa, H. Kiyama, et al. “Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1.J Biol Chem 276, no. 13 (March 30, 2001): 9688–98. https://doi.org/10.1074/jbc.M006153200.
Mitsuda N, Ohkubo N, Tamatani M, Lee YD, Taniguchi M, Namikawa K, et al. Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1. J Biol Chem. 2001 Mar 30;276(13):9688–98.
Mitsuda, N., et al. “Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1.J Biol Chem, vol. 276, no. 13, Mar. 2001, pp. 9688–98. Pubmed, doi:10.1074/jbc.M006153200.
Mitsuda N, Ohkubo N, Tamatani M, Lee YD, Taniguchi M, Namikawa K, Kiyama H, Yamaguchi A, Sato N, Sakata K, Ogihara T, Vitek MP, Tohyama M. Activated cAMP-response element-binding protein regulates neuronal expression of presenilin-1. J Biol Chem. 2001 Mar 30;276(13):9688–9698.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 30, 2001

Volume

276

Issue

13

Start / End Page

9688 / 9698

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Transcription, Genetic
  • Time Factors
  • Signal Transduction
  • Sequence Analysis, DNA
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Glutamate
  • Rats
  • RNA, Messenger