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Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells.

Publication ,  Journal Article
Gubits, RM; Yu, H; Casey, G; Munell, F; Vitek, MP
Published in: J Neurosci Res
October 1992

Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and beta-adrenergic regulation of gene expression in early (P39-47) and late (P55-90) passage C6 cells. Morphological changes were observed in five independently derived, late passage populations. In four of the five, the untreated cells were more polygonal than the fibroblast-like parental cells, and only a small fraction exhibited process outgrowth after dbcAMP treatment. Untreated cells from the fifth late passage population had longer cytoplasmic processes than parental cells and responded to dbcAMP with further process outgrowth. All late passage populations had shorter generation times than the parental cells. In early passage cells, treatment with the beta-adrenergic agonist, isoproterenol (IPR), resulted in an increase in c-fos mRNA and a decrease in c-jun mRNA (Gu-bits RM, Yu H: J Neurosci Res, 30:625-630, 1991). Both of these immediate early gene responses were irreversibly lost between P50 and P55. Additional differences in basal or IPR-induced mRNA levels were observed for beta-APP, GFAP, NGF, and PPE, but not for a number of other mRNAs. These results are discussed in relationship to previously described differences in the ability of early and late passage C6 cells to accumulate cAMP (Mallorga P, et al.: Biochim Biophys Acta 678:221-229, 1981).

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Published In

J Neurosci Res

DOI

ISSN

0360-4012

Publication Date

October 1992

Volume

33

Issue

2

Start / End Page

297 / 305

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Receptors, Adrenergic, beta
  • Rats
  • RNA, Neoplasm
  • RNA, Messenger
  • Protein Precursors
  • Neurology & Neurosurgery
  • Nerve Growth Factors
  • Kinetics
 

Citation

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Gubits, R. M., Yu, H., Casey, G., Munell, F., & Vitek, M. P. (1992). Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells. J Neurosci Res, 33(2), 297–305. https://doi.org/10.1002/jnr.490330213
Gubits, R. M., H. Yu, G. Casey, F. Munell, and M. P. Vitek. “Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells.J Neurosci Res 33, no. 2 (October 1992): 297–305. https://doi.org/10.1002/jnr.490330213.
Gubits RM, Yu H, Casey G, Munell F, Vitek MP. Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells. J Neurosci Res. 1992 Oct;33(2):297–305.
Gubits, R. M., et al. “Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells.J Neurosci Res, vol. 33, no. 2, Oct. 1992, pp. 297–305. Pubmed, doi:10.1002/jnr.490330213.
Gubits RM, Yu H, Casey G, Munell F, Vitek MP. Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells. J Neurosci Res. 1992 Oct;33(2):297–305.
Journal cover image

Published In

J Neurosci Res

DOI

ISSN

0360-4012

Publication Date

October 1992

Volume

33

Issue

2

Start / End Page

297 / 305

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Receptors, Adrenergic, beta
  • Rats
  • RNA, Neoplasm
  • RNA, Messenger
  • Protein Precursors
  • Neurology & Neurosurgery
  • Nerve Growth Factors
  • Kinetics