A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs.
Journal Article (Journal Article)
Using an S1 nuclease protection assay, we have identified a novel "variant" Amyloid Precursor Protein (APP) RNA in human brain which is 3-6-fold more abundant than APP-770, but less abundant than APP-751 or APP-695. This variant, referred to as amyloid precursor-related protein 365 (APRP-365), is not detected in mouse and rat brain RNAs. A 1.6 kilo-basepair cDNA clone corresponding to this variant APP RNA predicts the existence of a 365 amino acid protein that is similar to the amino-terminal end of APP-770 but lacks the beta-amyloid peptide and any hydrophobic transmembrane spanning domains. In a modified polymerase chain reaction (PCR), we used amplification of reverse transcribed mRNA to confirm and extend our S1 observations. Together, the features of APRP-365 suggest that the human variant is a soluble protein containing a Kunitz protease inhibitor domain.
Full Text
Duke Authors
Cited Authors
- Jacobsen, JS; Muenkel, HA; Blume, AJ; Vitek, MP
Published Date
- 1991
Published In
Volume / Issue
- 12 / 5
Start / End Page
- 575 - 583
PubMed ID
- 1722873
International Standard Serial Number (ISSN)
- 0197-4580
Digital Object Identifier (DOI)
- 10.1016/0197-4580(91)90089-3
Language
- eng
Conference Location
- United States