Skip to main content
Journal cover image

A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs.

Publication ,  Journal Article
Jacobsen, JS; Muenkel, HA; Blume, AJ; Vitek, MP
Published in: Neurobiol Aging
1991

Using an S1 nuclease protection assay, we have identified a novel "variant" Amyloid Precursor Protein (APP) RNA in human brain which is 3-6-fold more abundant than APP-770, but less abundant than APP-751 or APP-695. This variant, referred to as amyloid precursor-related protein 365 (APRP-365), is not detected in mouse and rat brain RNAs. A 1.6 kilo-basepair cDNA clone corresponding to this variant APP RNA predicts the existence of a 365 amino acid protein that is similar to the amino-terminal end of APP-770 but lacks the beta-amyloid peptide and any hydrophobic transmembrane spanning domains. In a modified polymerase chain reaction (PCR), we used amplification of reverse transcribed mRNA to confirm and extend our S1 observations. Together, the features of APRP-365 suggest that the human variant is a soluble protein containing a Kunitz protease inhibitor domain.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Neurobiol Aging

DOI

ISSN

0197-4580

Publication Date

1991

Volume

12

Issue

5

Start / End Page

575 / 583

Location

United States

Related Subject Headings

  • Templates, Genetic
  • Single-Strand Specific DNA and RNA Endonucleases
  • Rats
  • RNA-Directed DNA Polymerase
  • RNA, Messenger
  • RNA Splicing
  • RNA Probes
  • RNA
  • Polymerase Chain Reaction
  • Oligonucleotide Probes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jacobsen, J. S., Muenkel, H. A., Blume, A. J., & Vitek, M. P. (1991). A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs. Neurobiol Aging, 12(5), 575–583. https://doi.org/10.1016/0197-4580(91)90089-3
Jacobsen, J. S., H. A. Muenkel, A. J. Blume, and M. P. Vitek. “A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs.Neurobiol Aging 12, no. 5 (1991): 575–83. https://doi.org/10.1016/0197-4580(91)90089-3.
Jacobsen JS, Muenkel HA, Blume AJ, Vitek MP. A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs. Neurobiol Aging. 1991;12(5):575–83.
Jacobsen, J. S., et al. “A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs.Neurobiol Aging, vol. 12, no. 5, 1991, pp. 575–83. Pubmed, doi:10.1016/0197-4580(91)90089-3.
Jacobsen JS, Muenkel HA, Blume AJ, Vitek MP. A novel species-specific RNA related to alternatively spliced amyloid precursor protein mRNAs. Neurobiol Aging. 1991;12(5):575–583.
Journal cover image

Published In

Neurobiol Aging

DOI

ISSN

0197-4580

Publication Date

1991

Volume

12

Issue

5

Start / End Page

575 / 583

Location

United States

Related Subject Headings

  • Templates, Genetic
  • Single-Strand Specific DNA and RNA Endonucleases
  • Rats
  • RNA-Directed DNA Polymerase
  • RNA, Messenger
  • RNA Splicing
  • RNA Probes
  • RNA
  • Polymerase Chain Reaction
  • Oligonucleotide Probes