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Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain.

Publication ,  Journal Article
Heninger, C; Saito, N; Tallman, JF; Garrett, KM; Vitek, MP; Duman, RS; Gallager, DW
Published in: J Mol Neurosci
1990

Rats treated chronically with diazepam develop tolerance to diazepam effects and show changes in sensitivity of GABAergic systems. In order to investigate possible molecular mechanisms associated with these changes, we have evaluated the effects of acute and chronic diazepam treatment on levels of mRNA for the alpha 1 and beta 1 subunits of the GABAA receptor. Northern blots were hybridized with 32P-labeled GABA alpha 1 and beta 1 cDNA probes, and resulting bands were quantified by autoradiography and densitometry. Levels of alpha 1 mRNA were significantly decreased in cerebral cortex but not in cerebellum or hippocampus of chronic diazepam-treated rats. Acute diazepam treatment did not change levels of alpha 1 mRNA in any of the brain regions. Levels of beta 1 mRNA were examined by Northern blot analysis and also by solution hybridization analysis using a 32P-labeled riboprobe. Both methods showed that beta 1 mRNA was not significantly changed by chronic diazepam treatment. These results demonstrate a specific change in alpha 1 subunit that is associated with a state of altered GABA sensitivity and provide further support for the regional heterogeneity of chronic diazepam effects.

Duke Scholars

Published In

J Mol Neurosci

DOI

ISSN

0895-8696

Publication Date

1990

Volume

2

Issue

2

Start / End Page

101 / 107

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Receptors, GABA-A
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Nucleic Acid Hybridization
  • Neurology & Neurosurgery
  • Male
  • Down-Regulation
  • Diazepam
 

Citation

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MLA
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Heninger, C., Saito, N., Tallman, J. F., Garrett, K. M., Vitek, M. P., Duman, R. S., & Gallager, D. W. (1990). Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain. J Mol Neurosci, 2(2), 101–107. https://doi.org/10.1007/BF02876917
Heninger, C., N. Saito, J. F. Tallman, K. M. Garrett, M. P. Vitek, R. S. Duman, and D. W. Gallager. “Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain.J Mol Neurosci 2, no. 2 (1990): 101–7. https://doi.org/10.1007/BF02876917.
Heninger C, Saito N, Tallman JF, Garrett KM, Vitek MP, Duman RS, et al. Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain. J Mol Neurosci. 1990;2(2):101–7.
Heninger, C., et al. “Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain.J Mol Neurosci, vol. 2, no. 2, 1990, pp. 101–07. Pubmed, doi:10.1007/BF02876917.
Heninger C, Saito N, Tallman JF, Garrett KM, Vitek MP, Duman RS, Gallager DW. Effects of continuous diazepam administration on GABAA subunit mRNA in rat brain. J Mol Neurosci. 1990;2(2):101–107.
Journal cover image

Published In

J Mol Neurosci

DOI

ISSN

0895-8696

Publication Date

1990

Volume

2

Issue

2

Start / End Page

101 / 107

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Receptors, GABA-A
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Nucleic Acid Hybridization
  • Neurology & Neurosurgery
  • Male
  • Down-Regulation
  • Diazepam