Skip to main content
Journal cover image

Interleukin-1 stimulates the beta-amyloid precursor protein promoter.

Publication ,  Journal Article
Donnelly, RJ; Friedhoff, AJ; Beer, B; Blume, AJ; Vitek, MP
Published in: Cell Mol Neurobiol
December 1990

1. Amyloid plaques found in the brains of Alzheimer's diseased patients are composed of the 42 amino acid beta-amyloid peptide (BAP) which is processed out of the larger amyloid precursor protein (APP). 2. To study the regulation of the APP gene expression, we have isolated the promoter region of this angle of this single-copy gene and produced a reporter gene system to determine if the promoter is responsive to agents that may cause the overproduction of APP leading to the abnormal accumulation of plaques in AD. 3. The promoter contains sequences homologous to heat shock elements, AP-1 binding sites, and phorbol ester-inducible sequences as well as GG-rich regions found in other constitutively expressed genes. 4. We show here that this promoter is inducible in cultured cells by interleukin-1 (IL-1) in a transient assay system and that the HSE and AP-1 binding site are required for this inducibility. 5. This induction of transcription from the APP promoter implies that this gene is responsive to tropic and/or trophic agents which may be present in the diseased brain.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell Mol Neurobiol

DOI

ISSN

0272-4340

Publication Date

December 1990

Volume

10

Issue

4

Start / End Page

485 / 495

Location

United States

Related Subject Headings

  • Up-Regulation
  • Recombinant Proteins
  • Protein Precursors
  • Promoter Regions, Genetic
  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Mice
  • Interleukin-1
  • Humans
  • Genes, Synthetic
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Donnelly, R. J., Friedhoff, A. J., Beer, B., Blume, A. J., & Vitek, M. P. (1990). Interleukin-1 stimulates the beta-amyloid precursor protein promoter. Cell Mol Neurobiol, 10(4), 485–495. https://doi.org/10.1007/BF00712843
Donnelly, R. J., A. J. Friedhoff, B. Beer, A. J. Blume, and M. P. Vitek. “Interleukin-1 stimulates the beta-amyloid precursor protein promoter.Cell Mol Neurobiol 10, no. 4 (December 1990): 485–95. https://doi.org/10.1007/BF00712843.
Donnelly RJ, Friedhoff AJ, Beer B, Blume AJ, Vitek MP. Interleukin-1 stimulates the beta-amyloid precursor protein promoter. Cell Mol Neurobiol. 1990 Dec;10(4):485–95.
Donnelly, R. J., et al. “Interleukin-1 stimulates the beta-amyloid precursor protein promoter.Cell Mol Neurobiol, vol. 10, no. 4, Dec. 1990, pp. 485–95. Pubmed, doi:10.1007/BF00712843.
Donnelly RJ, Friedhoff AJ, Beer B, Blume AJ, Vitek MP. Interleukin-1 stimulates the beta-amyloid precursor protein promoter. Cell Mol Neurobiol. 1990 Dec;10(4):485–495.
Journal cover image

Published In

Cell Mol Neurobiol

DOI

ISSN

0272-4340

Publication Date

December 1990

Volume

10

Issue

4

Start / End Page

485 / 495

Location

United States

Related Subject Headings

  • Up-Regulation
  • Recombinant Proteins
  • Protein Precursors
  • Promoter Regions, Genetic
  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Mice
  • Interleukin-1
  • Humans
  • Genes, Synthetic