Single-cell monitoring using fiberoptic nanosensors.

Journal Article (Review;Journal Article)

This article is a review on the design, fabrication, and applications of fiberoptic nanosensors for in vivo monitoring of individual living cells. The nanosensors were fabricated with tapered optical fibers with distal ends having nanometer-sized diameters. Bioreceptors, such as antibody, peptides, and nucleic acids, are immobilized on the fiber tips and designed to be selective to target analyte molecules of interest. A laser beam is transmitted into the fiber, producing an evanescent field at the tip of the nanofiber that is used to excite target molecules bound to the bioreceptor molecules. The fluorescence originated from the analyte molecules is detected by a photo-detection system. The advantages and limitations of nanosensors in providing minimally invasive tools to probe subcellular compartments inside individual living cells for health effect studies and medical applications are discussed in detail.

Full Text

Duke Authors

Cited Authors

  • Vo-Dinh, T; Zhang, Y

Published Date

  • January 2011

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 79 - 85

PubMed ID

  • 20677238

Electronic International Standard Serial Number (EISSN)

  • 1939-0041

International Standard Serial Number (ISSN)

  • 1939-5116

Digital Object Identifier (DOI)

  • 10.1002/wnan.112


  • eng