Skip to main content
Journal cover image

Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene.

Publication ,  Journal Article
Wabuyele, MB; Yan, F; Vo-Dinh, T
Published in: Analytical and bioanalytical chemistry
September 2010

This paper describes the application of plasmonics-based nanoprobes that combine the modulation of the plasmonics effect to change the surface-enhanced Raman scattering (SERS) of a Raman label and the specificity of a DNA hairpin loop sequence to recognize and discriminate a variety of molecular target sequences. Hybridization with target DNA opens the hairpin and physically separates the Raman label from the metal nanoparticle thus reducing the plasmonics effect and quenching the SERS signal of the label. We have successfully demonstrated the specificity and selectivity of the nanoprobes in the detection of a single-nucleotide polymorphism (SNP) in the breast cancer BRCA1 gene in a homogenous solution at room temperature. In addition, the potential application of plasmonics nanoprobes for quantitative DNA diagnostic testing is discussed.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Analytical and bioanalytical chemistry

DOI

EISSN

1618-2650

ISSN

1618-2642

Publication Date

September 2010

Volume

398

Issue

2

Start / End Page

729 / 736

Related Subject Headings

  • Spectrum Analysis, Raman
  • Sensitivity and Specificity
  • Polymorphism, Single Nucleotide
  • Nucleic Acid Hybridization
  • Humans
  • Genes, BRCA1
  • Female
  • DNA
  • Breast Neoplasms
  • Biosensing Techniques
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wabuyele, M. B., Yan, F., & Vo-Dinh, T. (2010). Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene. Analytical and Bioanalytical Chemistry, 398(2), 729–736. https://doi.org/10.1007/s00216-010-3992-1
Wabuyele, Musundi B., Fei Yan, and Tuan Vo-Dinh. “Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene.Analytical and Bioanalytical Chemistry 398, no. 2 (September 2010): 729–36. https://doi.org/10.1007/s00216-010-3992-1.
Wabuyele MB, Yan F, Vo-Dinh T. Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene. Analytical and bioanalytical chemistry. 2010 Sep;398(2):729–36.
Wabuyele, Musundi B., et al. “Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene.Analytical and Bioanalytical Chemistry, vol. 398, no. 2, Sept. 2010, pp. 729–36. Epmc, doi:10.1007/s00216-010-3992-1.
Wabuyele MB, Yan F, Vo-Dinh T. Plasmonics nanoprobes: detection of single-nucleotide polymorphisms in the breast cancer BRCA1 gene. Analytical and bioanalytical chemistry. 2010 Sep;398(2):729–736.
Journal cover image

Published In

Analytical and bioanalytical chemistry

DOI

EISSN

1618-2650

ISSN

1618-2642

Publication Date

September 2010

Volume

398

Issue

2

Start / End Page

729 / 736

Related Subject Headings

  • Spectrum Analysis, Raman
  • Sensitivity and Specificity
  • Polymorphism, Single Nucleotide
  • Nucleic Acid Hybridization
  • Humans
  • Genes, BRCA1
  • Female
  • DNA
  • Breast Neoplasms
  • Biosensing Techniques