Skip to main content
Journal cover image

Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene.

Publication ,  Journal Article
Culha, M; Stokes, D; Vo-Dinh, T
Published in: Expert review of molecular diagnostics
September 2003

A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Expert review of molecular diagnostics

DOI

EISSN

1744-8352

ISSN

1473-7159

Publication Date

September 2003

Volume

3

Issue

5

Start / End Page

669 / 675

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Silver
  • Rhodamines
  • Nucleic Acid Hybridization
  • Neoplasms
  • Molecular Structure
  • Humans
  • Genes, bcl-2
  • Fluorescent Dyes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Culha, M., Stokes, D., & Vo-Dinh, T. (2003). Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene. Expert Review of Molecular Diagnostics, 3(5), 669–675. https://doi.org/10.1586/14737159.3.5.669
Culha, Mustafa, David Stokes, and Tuan Vo-Dinh. “Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene.Expert Review of Molecular Diagnostics 3, no. 5 (September 2003): 669–75. https://doi.org/10.1586/14737159.3.5.669.
Culha M, Stokes D, Vo-Dinh T. Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene. Expert review of molecular diagnostics. 2003 Sep;3(5):669–75.
Culha, Mustafa, et al. “Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene.Expert Review of Molecular Diagnostics, vol. 3, no. 5, Sept. 2003, pp. 669–75. Epmc, doi:10.1586/14737159.3.5.669.
Culha M, Stokes D, Vo-Dinh T. Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene. Expert review of molecular diagnostics. 2003 Sep;3(5):669–675.
Journal cover image

Published In

Expert review of molecular diagnostics

DOI

EISSN

1744-8352

ISSN

1473-7159

Publication Date

September 2003

Volume

3

Issue

5

Start / End Page

669 / 675

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Silver
  • Rhodamines
  • Nucleic Acid Hybridization
  • Neoplasms
  • Molecular Structure
  • Humans
  • Genes, bcl-2
  • Fluorescent Dyes