Surface-enhanced Raman scattering for cancer diagnostics: detection of the BCL2 gene.

Journal Article (Journal Article)

A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

Full Text

Duke Authors

Cited Authors

  • Culha, M; Stokes, D; Vo-Dinh, T

Published Date

  • September 2003

Published In

Volume / Issue

  • 3 / 5

Start / End Page

  • 669 - 675

PubMed ID

  • 14510186

Electronic International Standard Serial Number (EISSN)

  • 1744-8352

International Standard Serial Number (ISSN)

  • 1473-7159

Digital Object Identifier (DOI)

  • 10.1586/14737159.3.5.669


  • eng