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Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors.

Publication ,  Journal Article
Cullum, BM; Griffin, GD; Miller, GH; Vo-Dinh, T
Published in: Analytical biochemistry
January 2000

Submicrometer fiber-optic biosensors have been developed and used to measure toxic chemicals within single cells. Optical fibers that have been pulled to a distal-end diameter of less than 1 micrometer are coated with antibodies to selectively bind the species of interest. This paper describes the use of these fibers to selectively measure the concentration of benzo[a]pyrene tetrol (BPT), a metabolite of benzo[a]pyrene, within individual cells of two different cell lines, human mammary carcinoma cells and rat liver epithelial cells. The results from these measurements have been used to determine the sensitivity, reproducibility, and usefulness of these nanosensors. The detection limit of these biosensors has been determined to be 0.64 +/- 0.17 x 10(-11) M for BPT.

Duke Scholars

Published In

Analytical biochemistry

DOI

EISSN

1096-0309

ISSN

0003-2697

Publication Date

January 2000

Volume

277

Issue

1

Start / End Page

25 / 32

Related Subject Headings

  • Tumor Cells, Cultured
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Rats
  • Pyrenes
  • Optical Fibers
  • Microscopy, Electron, Scanning
  • Liver
  • Humans
  • Fiber Optic Technology
 

Citation

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Cullum, B. M., Griffin, G. D., Miller, G. H., & Vo-Dinh, T. (2000). Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors. Analytical Biochemistry, 277(1), 25–32. https://doi.org/10.1006/abio.1999.4341
Cullum, B. M., G. D. Griffin, G. H. Miller, and T. Vo-Dinh. “Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors.Analytical Biochemistry 277, no. 1 (January 2000): 25–32. https://doi.org/10.1006/abio.1999.4341.
Cullum BM, Griffin GD, Miller GH, Vo-Dinh T. Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors. Analytical biochemistry. 2000 Jan;277(1):25–32.
Cullum, B. M., et al. “Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors.Analytical Biochemistry, vol. 277, no. 1, Jan. 2000, pp. 25–32. Epmc, doi:10.1006/abio.1999.4341.
Cullum BM, Griffin GD, Miller GH, Vo-Dinh T. Intracellular measurements in mammary carcinoma cells using fiber-optic nanosensors. Analytical biochemistry. 2000 Jan;277(1):25–32.
Journal cover image

Published In

Analytical biochemistry

DOI

EISSN

1096-0309

ISSN

0003-2697

Publication Date

January 2000

Volume

277

Issue

1

Start / End Page

25 / 32

Related Subject Headings

  • Tumor Cells, Cultured
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Rats
  • Pyrenes
  • Optical Fibers
  • Microscopy, Electron, Scanning
  • Liver
  • Humans
  • Fiber Optic Technology