Differentiation of normal and neoplastic cells by synchronous fluorescence: Rat liver epithelial and rat hepatoma cell models


Journal Article

In the present study, conventional and synchronous luminescence (SL) were utilized to investigate spectral differences in normal and neoplastic cells. The synchronous fluorescence (SE) method involves scanning simultaneously both emission and excitation wavelengths while keeping a constant wavelength interval between them. This SE procedure simplifies the emission spectrum and provides for greater selectivity and is used to detect subtle differences in the fluorescence emission of the biochemical species of cells from rat tissues. A difference between the fluorescent spectra of the normal rat liver epithelial (RLE) and hepatoma cell lines were detected using synchronous fluorescence. The potential use of SE as a screening tool for cancer diagnosis is discussed.

Full Text

Duke Authors

Cited Authors

  • Watts, WE; Isola, NR; Frazier, D; Vo-Dinh, T

Published Date

  • January 1, 1999

Published In

Volume / Issue

  • 32 / 13

Start / End Page

  • 2583 - 2594

International Standard Serial Number (ISSN)

  • 0003-2719

Digital Object Identifier (DOI)

  • 10.1080/00032719908542989

Citation Source

  • Scopus