Gel-Based Indo-1 Probe for Monitoring Calcium(II) Ions

This work describes the development of a new gel-based Indo-1 (GBI) fluorescent probe for use in detection of Ca2+. The carbodiimide-binding procedure, which allows the formation of an amide bond between the aromatic carboxylic group of Indo-1 and an amino group of the gel has been selected for this study. In this work, agarose gel, which has an amino terminal group with a six-atom hydrophilic spacer arm, has been chosen for hosting Indo-1. A phase-transfer catalyst was used to allow efficient diffusion of metal ions into the GBI probe. The results indicate that the GBI probe is an efficient and practical system that has the required sensitivity to detect Ca2+ ions in biological fluids for routine monitoring. © 1994, American Chemical Society. All rights reserved.

Duke Scholars

Author Tuan Vo-Dinh Biomedical Engineering