Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples.


Journal Article

This paper presents the application of a fiber-optic antibody-based fluoroimmunosensor (FIS) for measuring DNA adducts of benzo[alpha]pyrene in human placenta samples. The FIS combines the excellent specificity of the antigen-antibody reaction, the high sensitivity of laser excitation, and the versatility of fiber-optic technology. The FIS was used to detect benzo[alpha]pyrene tetrol (BPT) after release from DNA by mild acid hydrolysis. These placenta samples contained one molecule of adduct per 10(7) base pairs. The limit of detection of the FIS for BPT is about 14 amol (14 x 10(-18) mol).

Full Text

Duke Authors

Cited Authors

  • Vo-Dinh, T; Alarie, JP; Johnson, RW; Sepaniak, MJ; Santella, RM

Published Date

  • April 1991

Published In

Volume / Issue

  • 37 / 4

Start / End Page

  • 532 - 535

PubMed ID

  • 2015666

Pubmed Central ID

  • 2015666

Electronic International Standard Serial Number (EISSN)

  • 1530-8561

International Standard Serial Number (ISSN)

  • 0009-9147


  • eng