Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples.
Publication
, Journal Article
Vo-Dinh, T; Alarie, JP; Johnson, RW; Sepaniak, MJ; Santella, RM
Published in: Clinical chemistry
April 1991
This paper presents the application of a fiber-optic antibody-based fluoroimmunosensor (FIS) for measuring DNA adducts of benzo[alpha]pyrene in human placenta samples. The FIS combines the excellent specificity of the antigen-antibody reaction, the high sensitivity of laser excitation, and the versatility of fiber-optic technology. The FIS was used to detect benzo[alpha]pyrene tetrol (BPT) after release from DNA by mild acid hydrolysis. These placenta samples contained one molecule of adduct per 10(7) base pairs. The limit of detection of the FIS for BPT is about 14 amol (14 x 10(-18) mol).
Duke Scholars
Published In
Clinical chemistry
EISSN
1530-8561
ISSN
0009-9147
Publication Date
April 1991
Volume
37
Issue
4
Start / End Page
532 / 535
Related Subject Headings
- Placenta
- Optical Fibers
- Humans
- General Clinical Medicine
- Fluoroimmunoassay
- Fiber Optic Technology
- Female
- Evaluation Studies as Topic
- Environmental Exposure
- DNA
Citation
APA
Chicago
ICMJE
MLA
NLM
Vo-Dinh, T., Alarie, J. P., Johnson, R. W., Sepaniak, M. J., & Santella, R. M. (1991). Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples. Clinical Chemistry, 37(4), 532–535.
Vo-Dinh, T., J. P. Alarie, R. W. Johnson, M. J. Sepaniak, and R. M. Santella. “Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples.” Clinical Chemistry 37, no. 4 (April 1991): 532–35.
Vo-Dinh T, Alarie JP, Johnson RW, Sepaniak MJ, Santella RM. Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples. Clinical chemistry. 1991 Apr;37(4):532–5.
Vo-Dinh, T., et al. “Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples.” Clinical Chemistry, vol. 37, no. 4, Apr. 1991, pp. 532–35.
Vo-Dinh T, Alarie JP, Johnson RW, Sepaniak MJ, Santella RM. Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples. Clinical chemistry. 1991 Apr;37(4):532–535.
Published In
Clinical chemistry
EISSN
1530-8561
ISSN
0009-9147
Publication Date
April 1991
Volume
37
Issue
4
Start / End Page
532 / 535
Related Subject Headings
- Placenta
- Optical Fibers
- Humans
- General Clinical Medicine
- Fluoroimmunoassay
- Fiber Optic Technology
- Female
- Evaluation Studies as Topic
- Environmental Exposure
- DNA