Fluorescence monitoring of a benzo[a]pyrene metabolite using a regenerable immunochemical-based fiber-optic sensor


Journal Article

Microscale regenerable biosensors are described and utilized to measure the natural fluorophor benzo[a]pyrene tetraol (BPT). The sensors combine laser-excited/fiber-optic remote sensing principles with a unique capillary tube delivery system to make repetitive, heterogeneous fluoroimmunoassay measurements. Two sensor configurations and modes of operation are described. Concentrations of BPT in the nanomolar range are easily measured with a reproducibility of 10% or better, depending on the sensor design, selective measurements can be made in ca. 20 min, then the sensor can be regenerated by delivering new reagents to the sensing chamber, without removing the sensor from the sample. © 1990.

Full Text

Duke Authors

Cited Authors

  • Alarie, JP; Bowyer, JR; Sepaniak, MJ; Hoyt, AM; Vo-dinh, T

Published Date

  • January 1, 1990

Published In

Volume / Issue

  • 236 / C

Start / End Page

  • 237 - 244

International Standard Serial Number (ISSN)

  • 0003-2670

Digital Object Identifier (DOI)

  • 10.1016/S0003-2670(00)83317-6

Citation Source

  • Scopus