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Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins.

Publication ,  Journal Article
Bardwell, L; Cook, JG; Zhu-Shimoni, JX; Voora, D; Thorner, J
Published in: Proc Natl Acad Sci U S A
December 22, 1998

Kss1, a yeast mitogen-activated protein kinase (MAPK), in its unphosphorylated (unactivated) state binds directly to and represses Ste12, a transcription factor necessary for expression of genes whose promoters contain filamentous response elements (FREs) and genes whose promoters contain pheromone response elements (PREs). Herein we show that two nuclear proteins, Dig1 and Dig2, are required cofactors in Kss1-imposed repression. Dig1 and Dig2 cooperate with Kss1 to repress Ste12 action at FREs and regulate invasive growth in a naturally invasive strain. Kss1-imposed Dig-dependent repression of Ste12 also occurs at PREs. However, maintenance of repression at PREs is more dependent on Dig1 and/or Dig2 and less dependent on Kss1 than repression at FREs. In addition, derepression at PREs is more dependent on MAPK-mediated phosphorylation than is derepression at FREs. Differential utilization of two types of MAPK-mediated regulation (binding-imposed repression and phosphorylation-dependent activation), in combination with distinct Ste12-containing complexes, contributes to the mechanisms by which separate extracellular stimuli that use the same MAPK cascade can elicit two different transcriptional responses.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 22, 1998

Volume

95

Issue

26

Start / End Page

15400 / 15405

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Proteins
  • Promoter Regions, Genetic
  • Phosphorylation
  • Pheromones
  • Models, Biological
  • Mitogen-Activated Protein Kinases
 

Citation

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Bardwell, L., Cook, J. G., Zhu-Shimoni, J. X., Voora, D., & Thorner, J. (1998). Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A, 95(26), 15400–15405. https://doi.org/10.1073/pnas.95.26.15400
Bardwell, L., J. G. Cook, J. X. Zhu-Shimoni, D. Voora, and J. Thorner. “Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins.Proc Natl Acad Sci U S A 95, no. 26 (December 22, 1998): 15400–405. https://doi.org/10.1073/pnas.95.26.15400.
Bardwell L, Cook JG, Zhu-Shimoni JX, Voora D, Thorner J. Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15400–5.
Bardwell, L., et al. “Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins.Proc Natl Acad Sci U S A, vol. 95, no. 26, Dec. 1998, pp. 15400–05. Pubmed, doi:10.1073/pnas.95.26.15400.
Bardwell L, Cook JG, Zhu-Shimoni JX, Voora D, Thorner J. Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15400–15405.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 22, 1998

Volume

95

Issue

26

Start / End Page

15400 / 15405

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Proteins
  • Promoter Regions, Genetic
  • Phosphorylation
  • Pheromones
  • Models, Biological
  • Mitogen-Activated Protein Kinases