A dichotomy of protection by bcl-2 from mitochondrial poisons
Bcl-2 is a primarily mitochondrial protein that represses programmed cell death. We find that cells overexpressing Bcl-2 are protected from death induced by treatment agents that induce Reactive Oxygen Species, such as 95% oxygen, H2O2, tBOOH, BSO, doxorubicin and xanthine + xanthine oxidase. However Bcl-2 did not protect cells treated with the respiratory chain inhibitors MPP+, rotenone, 3-NPA, antimycin A and cyanide. Thus while oxidants trigger programmed cell death, acute poisons of the electron transport chain kill cells by a Bcl-2 independent mechanism.
Hutching, TP; Wang, E; Bredesen, D; Cortopassil, GA
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