The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons.

Published online

Journal Article

Nociceptors are a subset of small primary afferent neurons that respond to noxious chemical, thermal and mechanical stimuli. Ion channels in nociceptors respond differently to noxious stimuli and generate electrical signals in different ways. Anoctamin 1 (ANO1 also known as TMEM16A) is a Ca(2+)-activated chloride channel that is essential for numerous physiological functions. We found that ANO1 was activated by temperatures over 44 °C with steep heat sensitivity. ANO1 was expressed in small sensory neurons and was highly colocalized with nociceptor markers, which suggests that it may be involved in nociception. Application of heat ramps to dorsal root ganglion (DRG) neurons elicited robust ANO1-dependent depolarization. Furthermore, knockdown or deletion of ANO1 in DRG neurons substantially reduced nociceptive behavior in thermal pain models. These results indicate that ANO1 is a heat sensor that detects nociceptive thermal stimuli in sensory neurons and possibly mediates nociception.

Full Text

Duke Authors

Cited Authors

  • Cho, H; Yang, YD; Lee, J; Lee, B; Kim, T; Jang, Y; Back, SK; Na, HS; Harfe, BD; Wang, F; Raouf, R; Wood, JN; Oh, U

Published Date

  • May 27, 2012

Published In

Volume / Issue

  • 15 / 7

Start / End Page

  • 1015 - 1021

PubMed ID

  • 22634729

Pubmed Central ID

  • 22634729

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

Digital Object Identifier (DOI)

  • 10.1038/nn.3111


  • eng

Conference Location

  • United States