SLIT2-mediated ROBO2 signaling restricts kidney induction to a single site.

Published

Journal Article

Kidney development occurs in a stereotypic position along the body axis. It begins when a single ureteric bud emerges from the nephric duct in response to GDNF secreted by the adjacent nephrogenic mesenchyme. Posterior restriction of Gdnf expression is considered critical for correct positioning of ureteric bud development. Here we show that mouse mutants lacking either SLIT2 or its receptor ROBO2, molecules known primarily for their function in axon guidance and cell migration, develop supernumerary ureteric buds that remain inappropriately connected to the nephric duct, and that the SLIT2/ROBO2 signal is transduced in the nephrogenic mesenchyme. Furthermore, we show that Gdnf expression is inappropriately maintained in anterior nephrogenic mesenchyme in these mutants. Thus our data identify an intercellular signaling system that restricts, directly or indirectly, the extent of the Gdnf expression domain, thereby precisely positioning the site of kidney induction.

Full Text

Duke Authors

Cited Authors

  • Grieshammer, U; Le Ma, ; Plump, AS; Wang, F; Tessier-Lavigne, M; Martin, GR

Published Date

  • May 2004

Published In

Volume / Issue

  • 6 / 5

Start / End Page

  • 709 - 717

PubMed ID

  • 15130495

Pubmed Central ID

  • 15130495

International Standard Serial Number (ISSN)

  • 1534-5807

Language

  • eng

Conference Location

  • United States