In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.

Journal Article (Journal Article)

We performed epi-mode pump-probe imaging of melanin in excised human pigmented lesions and both hemoglobin and melanin in live xenograft mouse melanoma models to depths greater than 100 µm. Eumelanin and pheomelanin images, which have been previously demonstrated to differentiate melanoma from benign lesions, were acquired at the dermal-epidermal junction with cellular resolution and modest optical powers (down to 15 mW). We imaged dermal microvasculature with the same wavelengths, allowing simultaneous acquisition of melanin, hemoglobin and multiphoton autofluorescence images. Molecular pump-probe imaging of melanocytes, skin structure and microvessels allows comprehensive, non-invasive characterization of pigmented lesions.

Full Text

Duke Authors

Cited Authors

  • Matthews, TE; Wilson, JW; Degan, S; Simpson, MJ; Jin, JY; Zhang, JY; Warren, WS

Published Date

  • June 1, 2011

Published In

Volume / Issue

  • 2 / 6

Start / End Page

  • 1576 - 1583

PubMed ID

  • 21698020

Pubmed Central ID

  • PMC3114225

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.2.001576


  • eng

Conference Location

  • United States