In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.
Publication
, Journal Article
Matthews, TE; Wilson, JW; Degan, S; Simpson, MJ; Jin, JY; Zhang, JY; Warren, WS
Published in: Biomed Opt Express
June 1, 2011
We performed epi-mode pump-probe imaging of melanin in excised human pigmented lesions and both hemoglobin and melanin in live xenograft mouse melanoma models to depths greater than 100 µm. Eumelanin and pheomelanin images, which have been previously demonstrated to differentiate melanoma from benign lesions, were acquired at the dermal-epidermal junction with cellular resolution and modest optical powers (down to 15 mW). We imaged dermal microvasculature with the same wavelengths, allowing simultaneous acquisition of melanin, hemoglobin and multiphoton autofluorescence images. Molecular pump-probe imaging of melanocytes, skin structure and microvessels allows comprehensive, non-invasive characterization of pigmented lesions.
Duke Scholars
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Published In
Biomed Opt Express
DOI
EISSN
2156-7085
Publication Date
June 1, 2011
Volume
2
Issue
6
Start / End Page
1576 / 1583
Location
United States
Related Subject Headings
- 5102 Atomic, molecular and optical physics
- 4003 Biomedical engineering
- 3212 Ophthalmology and optometry
- 0912 Materials Engineering
- 0205 Optical Physics
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Matthews, T. E., Wilson, J. W., Degan, S., Simpson, M. J., Jin, J. Y., Zhang, J. Y., & Warren, W. S. (2011). In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. Biomed Opt Express, 2(6), 1576–1583. https://doi.org/10.1364/BOE.2.001576
Matthews, Thomas E., Jesse W. Wilson, Simone Degan, Mary Jane Simpson, Jane Y. Jin, Jennifer Y. Zhang, and Warren S. Warren. “In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.” Biomed Opt Express 2, no. 6 (June 1, 2011): 1576–83. https://doi.org/10.1364/BOE.2.001576.
Matthews TE, Wilson JW, Degan S, Simpson MJ, Jin JY, Zhang JY, et al. In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. Biomed Opt Express. 2011 Jun 1;2(6):1576–83.
Matthews, Thomas E., et al. “In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature.” Biomed Opt Express, vol. 2, no. 6, June 2011, pp. 1576–83. Pubmed, doi:10.1364/BOE.2.001576.
Matthews TE, Wilson JW, Degan S, Simpson MJ, Jin JY, Zhang JY, Warren WS. In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. Biomed Opt Express. 2011 Jun 1;2(6):1576–1583.
Published In
Biomed Opt Express
DOI
EISSN
2156-7085
Publication Date
June 1, 2011
Volume
2
Issue
6
Start / End Page
1576 / 1583
Location
United States
Related Subject Headings
- 5102 Atomic, molecular and optical physics
- 4003 Biomedical engineering
- 3212 Ophthalmology and optometry
- 0912 Materials Engineering
- 0205 Optical Physics