Probing skin pigmentation changes with transient absorption imaging of eumelanin and pheomelanin.

Published

Journal Article

As some of the most ubiquitous and biologically important natural pigments, melanins play essential roles in the photoprotection of skin. Changes in melanin production could potentially be useful for clinical diagnosis of the progression stage of melanoma. Previously we demonstrated a new method for imaging melanin distribution in tissue with two-color transient absorption microscopy. Here we extend this study to longer wavelengths and show that we are able to image melanin in fixed thin skin slices with higher signal-to-noise ratios (SNRs) and demonstrate epimode imaging. We show that both photothermal effects and long-lived excited states can contribute to the long-lived signal. Eumelanin and pheomelanin exhibit markedly different long-lived excited state absorption. This difference should enable us to map out their respective distribution in tissue samples with subcellular resolution. This technique could provide valuable information in diagnosing the malignant transformation of melanocytes.

Full Text

Duke Authors

Cited Authors

  • Fu, D; Ye, T; Matthews, TE; Grichnik, J; Hong, L; Simon, JD; Warren, WS

Published Date

  • September 2008

Published In

Volume / Issue

  • 13 / 5

Start / End Page

  • 054036 -

PubMed ID

  • 19021416

Pubmed Central ID

  • 19021416

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.2976424

Language

  • eng