Generation of impossible cross-peaks between bulk water and biomolecules in solution NMR.
Published
Journal Article
Intermolecular multiple-quantum coherences between bulk water and a glycoprotein fragment at modest concentration (20 mM) have been experimentally produced and detected, although such coherences are inconceivable in the normal theoretical framework of nuclear magnetic resonance. A density matrix treatment explains these results by including the long-range dipolar interaction between spins and by discarding the high-temperature approximation. These results imply that peak intensities (critical for structural determinations) can be distorted in many gradient experiments, and show that magic-angle gradients provide substantial improvements with reduced gradient strengths. They also suggest methods for contrast enhancement in magnetic resonance imaging.
Full Text
Duke Authors
Cited Authors
- Warren, WS; Richter, W; Andreotti, AH; Farmer, BT
Published Date
- December 1993
Published In
Volume / Issue
- 262 / 5142
Start / End Page
- 2005 - 2009
PubMed ID
- 8266096
Pubmed Central ID
- 8266096
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.8266096
Language
- eng