Generation of impossible cross-peaks between bulk water and biomolecules in solution NMR.


Journal Article

Intermolecular multiple-quantum coherences between bulk water and a glycoprotein fragment at modest concentration (20 mM) have been experimentally produced and detected, although such coherences are inconceivable in the normal theoretical framework of nuclear magnetic resonance. A density matrix treatment explains these results by including the long-range dipolar interaction between spins and by discarding the high-temperature approximation. These results imply that peak intensities (critical for structural determinations) can be distorted in many gradient experiments, and show that magic-angle gradients provide substantial improvements with reduced gradient strengths. They also suggest methods for contrast enhancement in magnetic resonance imaging.

Full Text

Duke Authors

Cited Authors

  • Warren, WS; Richter, W; Andreotti, AH; Farmer, BT

Published Date

  • December 1993

Published In

Volume / Issue

  • 262 / 5142

Start / End Page

  • 2005 - 2009

PubMed ID

  • 8266096

Pubmed Central ID

  • 8266096

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.8266096


  • eng