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Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.

Publication ,  Journal Article
Seekell, K; Price, H; Marinakos, S; Wax, A
Published in: Methods (San Diego, Calif.)
February 2012

Noble metal nanoparticles hold great potential as optical contrast agents due to a unique feature, known as the plasmon resonance, which produces enhanced scattering and absorption at specific frequencies. The plasmon resonance also provides a spectral tunability that is not often found in organic fluorophores or other labeling methods. The ability to functionalize these nanoparticles with antibodies has led to their development as contrast agents for molecular optical imaging. In this review article, we present methods for optimizing the spectral agility of these labels. We discuss synthesis of gold nanorods, a plasmonic nanoparticle in which the plasmonic resonance can be tuned during synthesis to provide imaging within the spectral window commonly utilized in biomedical applications. We describe recent advances in our group to functionalize gold and silver nanoparticles using distinct antibodies, including EGFR, HER-2 and IGF-1, selected for their relevance to tumor imaging. Finally, we present characterization of these nanoparticle labels to verify their spectral properties and molecular specificity.

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Published In

Methods (San Diego, Calif.)

DOI

EISSN

1095-9130

ISSN

1046-2023

Publication Date

February 2012

Volume

56

Issue

2

Start / End Page

310 / 316

Related Subject Headings

  • Silver
  • Sensitivity and Specificity
  • Receptors, Cell Surface
  • Nanotubes
  • Nanotechnology
  • Nanospheres
  • Metal Nanoparticles
  • Immunohistochemistry
  • Humans
  • Gold
 

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Seekell, K., Price, H., Marinakos, S., & Wax, A. (2012). Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis. Methods (San Diego, Calif.), 56(2), 310–316. https://doi.org/10.1016/j.ymeth.2011.08.017
Seekell, Kevin, Hillel Price, Stella Marinakos, and Adam Wax. “Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.Methods (San Diego, Calif.) 56, no. 2 (February 2012): 310–16. https://doi.org/10.1016/j.ymeth.2011.08.017.
Seekell K, Price H, Marinakos S, Wax A. Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis. Methods (San Diego, Calif). 2012 Feb;56(2):310–6.
Seekell, Kevin, et al. “Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.Methods (San Diego, Calif.), vol. 56, no. 2, Feb. 2012, pp. 310–16. Epmc, doi:10.1016/j.ymeth.2011.08.017.
Seekell K, Price H, Marinakos S, Wax A. Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis. Methods (San Diego, Calif). 2012 Feb;56(2):310–316.
Journal cover image

Published In

Methods (San Diego, Calif.)

DOI

EISSN

1095-9130

ISSN

1046-2023

Publication Date

February 2012

Volume

56

Issue

2

Start / End Page

310 / 316

Related Subject Headings

  • Silver
  • Sensitivity and Specificity
  • Receptors, Cell Surface
  • Nanotubes
  • Nanotechnology
  • Nanospheres
  • Metal Nanoparticles
  • Immunohistochemistry
  • Humans
  • Gold