Detection of dysplasia in Barrett's esophagus with in vivo depth-resolved nuclear morphology measurements.

Journal Article (Journal Article)

Background & aims

Patients with Barrett's esophagus (BE) show increased risk of developing esophageal adenocarcinoma and are routinely examined using upper endoscopy with biopsy to detect neoplastic changes. Angle-resolved low coherence interferometry (a/LCI) uses in vivo depth-resolved nuclear morphology measurements to detect dysplasia. We assessed the clinical utility of a/LCI in the endoscopic surveillance of patients with BE.


Consecutive patients undergoing routine surveillance upper endoscopy for BE were recruited at 2 endoscopy centers. A novel, endoscope-compatible a/LCI system measured the mean diameter and refractive index of cell nuclei in esophageal epithelium at 172 biopsy sites in 46 patients. At each site, an a/LCI measurement was correlated with a concurrent endoscopic biopsy specimen. Each biopsy specimen was assessed histologically and classified as normal, nondysplastic BE, indeterminate for dysplasia, low-grade dysplasia (LGD), or high-grade dysplasia (HGD). The a/LCI data from multiple depths were analyzed to evaluate its ability to differentiate dysplastic from nondysplastic tissue.


Pathology characterized 5 of the scanned sites as HGD, 8 as LGD, 75 as nondysplastic BE, 70 as normal tissue types, and 14 as indeterminate for dysplasia. The a/LCI nuclear size measurements separated dysplastic from nondysplastic tissue at a statistically significant (P < .001) level for the tissue segment 200 to 300 μm beneath the surface with an accuracy of 86% (147/172). A receiver operator characteristic analysis indicated an area under the curve of 0.91, and an optimized decision point gave 100% (13/13) sensitivity and 84% (134/159) specificity.


These preliminary data suggest a/LCI is accurate in detecting dysplasia in vivo in patients with BE.

Full Text

Duke Authors

Cited Authors

  • Terry, NG; Zhu, Y; Rinehart, MT; Brown, WJ; Gebhart, SC; Bright, S; Carretta, E; Ziefle, CG; Panjehpour, M; Galanko, J; Madanick, RD; Dellon, ES; Trembath, D; Bennett, A; Goldblum, JR; Overholt, BF; Woosley, JT; Shaheen, NJ; Wax, A

Published Date

  • January 2011

Published In

Volume / Issue

  • 140 / 1

Start / End Page

  • 42 - 50

PubMed ID

  • 20854820

Pubmed Central ID

  • PMC3008285

Electronic International Standard Serial Number (EISSN)

  • 1528-0012

International Standard Serial Number (ISSN)

  • 0016-5085

Digital Object Identifier (DOI)

  • 10.1053/j.gastro.2010.09.008


  • eng