Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.
Published
Journal Article
We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with camera spatial bandwidth consumption compared to previous off-axis approaches and thus can capture finer sample spatial details, given a limited spatial bandwidth of a specific digital camera. Additionally, due to the parallel acquisition mechanism, the approach is suitable for visualizing rapid dynamic processes, permitting an interferometric acquisition rate equal to the camera frame rate. The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms.
Full Text
Duke Authors
Cited Authors
- Shaked, NT; Newpher, TM; Ehlers, MD; Wax, A
Published Date
- May 2010
Published In
Volume / Issue
- 49 / 15
Start / End Page
- 2872 - 2878
PubMed ID
- 20490249
Pubmed Central ID
- 20490249
Electronic International Standard Serial Number (EISSN)
- 1539-4522
International Standard Serial Number (ISSN)
- 1559-128X
Digital Object Identifier (DOI)
- 10.1364/ao.49.002872
Language
- eng