Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells.


Journal Article

We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis interferometry and fewer measurements than traditional on-axis interferometry. Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microscopy of live human skin cancer cells.

Full Text

Duke Authors

Cited Authors

  • Shaked, NT; Zhu, Y; Rinehart, MT; Wax, A

Published Date

  • August 2009

Published In

Volume / Issue

  • 17 / 18

Start / End Page

  • 15585 - 15591

PubMed ID

  • 19724557

Pubmed Central ID

  • 19724557

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.17.015585


  • eng