Skip to main content

Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations.

Publication ,  Journal Article
Graf, RN; Robles, FE; Chen, X; Wax, A
Published in: Journal of biomedical optics
November 2009

We have developed a novel dual-window approach for spectroscopic optical coherence tomography (OCT) measurements and applied it to probe nuclear morphology in tissue samples drawn from the hamster cheek pouch carcinogenesis model. The dual-window approach enables high spectral and depth resolution simultaneously, allowing detection of spectral oscillations, which we isolate to determine the structure of cell nuclei in the basal layer of the epithelium. The measurements were executed with our parallel frequency domain OCT system, which uses light from a thermal source, providing high bandwidth and access to the visible portion of the spectrum. The structural measurements show a highly statistically significant difference between untreated (normal) and treated (hyperplastic/dysplastic) tissues, indicating the potential utility of this approach as a diagnostic method.

Duke Scholars

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

November 2009

Volume

14

Issue

6

Start / End Page

064030

Related Subject Headings

  • Tomography, Optical Coherence
  • Scattering, Radiation
  • Precancerous Conditions
  • Optics
  • Mouth Neoplasms
  • Mesocricetus
  • Male
  • Light
  • Epithelium
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Graf, R. N., Robles, F. E., Chen, X., & Wax, A. (2009). Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations. Journal of Biomedical Optics, 14(6), 064030. https://doi.org/10.1117/1.3269680
Graf, Robert N., Francisco E. Robles, Xiaoxin Chen, and Adam Wax. “Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations.Journal of Biomedical Optics 14, no. 6 (November 2009): 064030. https://doi.org/10.1117/1.3269680.
Graf, Robert N., et al. “Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillations.Journal of Biomedical Optics, vol. 14, no. 6, Nov. 2009, p. 064030. Epmc, doi:10.1117/1.3269680.

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

November 2009

Volume

14

Issue

6

Start / End Page

064030

Related Subject Headings

  • Tomography, Optical Coherence
  • Scattering, Radiation
  • Precancerous Conditions
  • Optics
  • Mouth Neoplasms
  • Mesocricetus
  • Male
  • Light
  • Epithelium
  • Disease Models, Animal