Phase dispersion optical tomography applied to study sub-cellular motions
Phase dispersion optical tomography (PDOT) technique that eliminates interferometer jitter noise was analyzed. The input light was a pair of overlapped beams of laser light at wavelength λ and 2λ. The Gelatin and water layer sandwiched between two cover slips induced an optical path change to indicate noticeable phase shifts in reflection from the bottom cover slip. The technique was applied to study onion slice and it was shown that the PDOT can be exploited for analysis of sub-cellular dynamics in biological tissues.