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Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform.

Publication ,  Journal Article
Gabitzsch, ES; Balint-Junior, JP; Xu, Y; Balcaitis, S; Sanders-Beer, B; Karl, J; Weinhold, KJ; Paessler, S; Jones, FR
Published in: Vaccine
November 26, 2012

Anti-vector immunity mitigates immune responses induced by recombinant adenovirus vector vaccines, limiting their prime-boost capabilities. We have developed a novel gene delivery and expression platform (Ad5 [E1-, E2b-]) that induces immune responses despite pre-existing and/or developed concomitant Ad5 immunity. In the present study, we evaluated if this new Ad5 platform could overcome the adverse condition of pre-existing Ad5 immunity to induce effective immune responses in prime-boost immunization regimens against two different infectious diseases in the same animal. Ad5 immune rhesus macaques (RM) were immunized multiple times with the Ad5 [E1-, E2b-] platform expressing antigens from simian immunodeficiency virus (SIV). Immunized RM developed cell-mediated immunity against SIV antigens Gag, Pol, Nef and Env as well as antibody against Env. Vaccinated and vector control RMs were challenged intra-rectally with homologous SIVmac239. During a 7-week follow-up, there was perturbation of SIV load in some immunized RM. At 7 weeks post-challenge, eight immunized animals (53%) did not have detectable SIV, compared to two RM controls (13%) (P<0.02; log-rank Mantel-Cox test). There was no correlation of protective MHC contributing to infection control. The RM without detectable circulating SIV, now hyper immune to Ad5, were then vaccinated with the same Ad5 [E1-, E2b-] platform expressing H1N1 influenza hemagglutinin (HA). Thirty days post Ad5 [E1-, E2b-]-HA vaccination, significant levels of influenza neutralizing antibody were induced in all animals that increased after an Ad5 [E1-, E2b-]-HA homologous boost. These data demonstrate the versatility of this new vector platform to immunize against two separate disease targets in the same animal despite the presence of immunity against the delivery platform, permitting homologous repeat immunizations with an Ad5 gene delivery platform.

Duke Scholars

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Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

November 26, 2012

Volume

30

Issue

50

Start / End Page

7265 / 7270

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Load
  • Vaccines, Synthetic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
  • Male
  • Macaca mulatta
  • Influenza Vaccines
  • Influenza A Virus, H1N1 Subtype
 

Citation

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Gabitzsch, E. S., Balint-Junior, J. P., Xu, Y., Balcaitis, S., Sanders-Beer, B., Karl, J., … Jones, F. R. (2012). Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform. Vaccine, 30(50), 7265–7270. https://doi.org/10.1016/j.vaccine.2012.09.058
Gabitzsch, Elizabeth S., Joseph P. Balint-Junior, Younong Xu, Stephanie Balcaitis, Brigitte Sanders-Beer, Julie Karl, Kent J. Weinhold, Slobodan Paessler, and Frank R. Jones. “Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform.Vaccine 30, no. 50 (November 26, 2012): 7265–70. https://doi.org/10.1016/j.vaccine.2012.09.058.
Gabitzsch ES, Balint-Junior JP, Xu Y, Balcaitis S, Sanders-Beer B, Karl J, et al. Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform. Vaccine. 2012 Nov 26;30(50):7265–70.
Gabitzsch, Elizabeth S., et al. “Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform.Vaccine, vol. 30, no. 50, Nov. 2012, pp. 7265–70. Pubmed, doi:10.1016/j.vaccine.2012.09.058.
Gabitzsch ES, Balint-Junior JP, Xu Y, Balcaitis S, Sanders-Beer B, Karl J, Weinhold KJ, Paessler S, Jones FR. Control of SIV infection and subsequent induction of pandemic H1N1 immunity in rhesus macaques using an Ad5 [E1-, E2b-] vector platform. Vaccine. 2012 Nov 26;30(50):7265–7270.
Journal cover image

Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

November 26, 2012

Volume

30

Issue

50

Start / End Page

7265 / 7270

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Load
  • Vaccines, Synthetic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
  • Male
  • Macaca mulatta
  • Influenza Vaccines
  • Influenza A Virus, H1N1 Subtype