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Vaccine-induced protection of chimpanzees against infection by a heterologous human immunodeficiency virus type 1.

Publication ,  Journal Article
Girard, M; Meignier, B; Barré-Sinoussi, F; Kieny, MP; Matthews, T; Muchmore, E; Nara, PL; Wei, Q; Rimsky, L; Weinhold, K
Published in: J Virol
October 1995

The extraordinary genetic diversity of human immunodeficiency virus type 1 (HIV-1) is a major problem to overcome in the development of an effective vaccine. In the most reliable animal model of HIV-1 infection, chimpanzees were immunized with various combinations of HIV-1 antigens, which were derived primarily from the surface glycoprotein, gp160, of HIV-1 strains LAI and MN. The immunogens also included a live recombinant canarypox virus expressing a gp160-MN protein. In one experiment, two chimpanzees were immunized multiple times; one animal received antigens derived only from HIV-1LAI, and the second animal received antigens from both HIV-1LAI and HIV-1MN. In another experiment, four chimpanzees were immunized in parallel a total of five times over 18 months; two animals received purified gp160 and V3-MN peptides, whereas the other two animals received the recombinant canarypox virus and gp160. At 3 months after the final booster, all immunized and naive control chimpanzees were challenged by intravenous inoculation of HIV-1SF2; therefore, the study represented an intrasubtype B heterologous virus challenge. Virologic and serologic follow-up showed that the controls and the two chimpanzees immunized with the live recombinant canarypox virus became infected, whereas the other animals that were immunized with gp160 and V3-MN peptides were protected from infection. Evaluation of both cellular and humoral HIV-specific immune responses at the time of infectious HIV-1 challenge identified the following as possible correlates of protection: antibody titers to the V3 loop of MN and neutralizing antibody titers to HIV-1MN or HIV-1LAI, but not to HIV-1SF2. The results of this study indicate that vaccine-mediated protection against intravenous infection with heterologous HIV-1 strains of the same subtype is possible with some immunogens.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

October 1995

Volume

69

Issue

10

Start / End Page

6239 / 6248

Location

United States

Related Subject Headings

  • Virology
  • Time Factors
  • Species Specificity
  • Polymerase Chain Reaction
  • Pan troglodytes
  • Molecular Sequence Data
  • Male
  • Immunoblotting
  • HIV-1
  • HIV Antigens
 

Citation

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Chicago
ICMJE
MLA
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Girard, M., Meignier, B., Barré-Sinoussi, F., Kieny, M. P., Matthews, T., Muchmore, E., … Weinhold, K. (1995). Vaccine-induced protection of chimpanzees against infection by a heterologous human immunodeficiency virus type 1. J Virol, 69(10), 6239–6248. https://doi.org/10.1128/JVI.69.10.6239-6248.1995
Girard, M., B. Meignier, F. Barré-Sinoussi, M. P. Kieny, T. Matthews, E. Muchmore, P. L. Nara, Q. Wei, L. Rimsky, and K. Weinhold. “Vaccine-induced protection of chimpanzees against infection by a heterologous human immunodeficiency virus type 1.J Virol 69, no. 10 (October 1995): 6239–48. https://doi.org/10.1128/JVI.69.10.6239-6248.1995.
Girard M, Meignier B, Barré-Sinoussi F, Kieny MP, Matthews T, Muchmore E, et al. Vaccine-induced protection of chimpanzees against infection by a heterologous human immunodeficiency virus type 1. J Virol. 1995 Oct;69(10):6239–48.
Girard, M., et al. “Vaccine-induced protection of chimpanzees against infection by a heterologous human immunodeficiency virus type 1.J Virol, vol. 69, no. 10, Oct. 1995, pp. 6239–48. Pubmed, doi:10.1128/JVI.69.10.6239-6248.1995.
Girard M, Meignier B, Barré-Sinoussi F, Kieny MP, Matthews T, Muchmore E, Nara PL, Wei Q, Rimsky L, Weinhold K. Vaccine-induced protection of chimpanzees against infection by a heterologous human immunodeficiency virus type 1. J Virol. 1995 Oct;69(10):6239–6248.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

October 1995

Volume

69

Issue

10

Start / End Page

6239 / 6248

Location

United States

Related Subject Headings

  • Virology
  • Time Factors
  • Species Specificity
  • Polymerase Chain Reaction
  • Pan troglodytes
  • Molecular Sequence Data
  • Male
  • Immunoblotting
  • HIV-1
  • HIV Antigens