Composition and organization of active centromere sequences in complex genomes.

Published

Journal Article

Centromeres are sites of chromosomal spindle attachment during mitosis and meiosis. While the sequence basis for centromere identity remains a subject of considerable debate, one approach is to examine the genomic organization at these active sites that are correlated with epigenetic marks of centromere function.We have developed an approach to characterize both satellite and non-satellite centromeric sequences that are missing from current assemblies in complex genomes, using the dog genome as an example. Combining this genomic reference with an epigenetic dataset corresponding to sequences associated with the histone H3 variant centromere protein A (CENP-A), we identify active satellite sequence domains that appear to be both functionally and spatially distinct within the overall definition of satellite families.These findings establish a genomic and epigenetic foundation for exploring the functional role of centromeric sequences in the previously sequenced dog genome and provide a model for similar studies within the context of less-characterized genomes.

Full Text

Cited Authors

  • Hayden, KE; Willard, HF

Published Date

  • July 20, 2012

Published In

Volume / Issue

  • 13 /

Start / End Page

  • 324 -

PubMed ID

  • 22817545

Pubmed Central ID

  • 22817545

Electronic International Standard Serial Number (EISSN)

  • 1471-2164

International Standard Serial Number (ISSN)

  • 1471-2164

Digital Object Identifier (DOI)

  • 10.1186/1471-2164-13-324

Language

  • eng