Apoptosis: final common pathway of photoreceptor death in rd, rds, and rhodopsin mutant mice.

Published

Journal Article

Mutations in the retinal degeneration, retinal degeneration slow(/peripherin) and rhodopsin genes cause photoreceptor degeneration in humans and mice. Although the phenotypes arising from these mutations are different, suggesting different mechanisms of pathogenesis, we present evidence that apoptosis may be the final common pathway of the disease process linking genotype to phenotype. We observed internucleosomal cleavage of retinal DNA by gel electrophoresis and fragmented DNA at the single cell level by labeling the nicked DNA ends with biotinylated poly(dU). In retinal degeneration mice, DNA fragmentation occurred during the period of photoreceptor degeneration. In retinal degeneration slow mice and in transgenic mice expressing a mutant (Pro347Ser) rhodopsin gene, DNA fragmentation occurred after normal histogenetic cell death (also apoptosis) had ceased. Since DNA fragmentation by internucleosomal cleavage is a cardinal feature of apoptosis, our data suggest that all three of these genetic mutations lead to apoptosis.

Full Text

Duke Authors

Cited Authors

  • Chang, GQ; Hao, Y; Wong, F

Published Date

  • October 1993

Published In

Volume / Issue

  • 11 / 4

Start / End Page

  • 595 - 605

PubMed ID

  • 8398150

Pubmed Central ID

  • 8398150

International Standard Serial Number (ISSN)

  • 0896-6273

Digital Object Identifier (DOI)

  • 10.1016/0896-6273(93)90072-y

Language

  • eng

Conference Location

  • United States