A DNA deletion associated with multiple impaired transcripts in the visual mutant TRP.

Published

Journal Article

The transient receptor potential (trp) mutant in Drosophila is known to manifest retinal degeneration and involves defects in the intermediate steps of visual transduction. The chromosome walking technique has been conducted at the cytogenetic location of trp. Overlapping phage clones that cover a DNA stretch of 73 kb were obtained. Southern blotting studies detected a 2.3 kb DNA deletion within this region in the mutant. The deleted DNA fragment contains exons of three transcripts with lengths of 3.5, 1.5 and 0.8 kb. The transcription of the 3.5 and 1.5 kb RNAs is completely abolished in the mutant, whereas that of 0.8 kb RNA appears to be modified. The impairment in production of three RNAs, due to the DNA deletion, offers a possible molecular basis of the multiple defects displayed by the trp- phenotype.

Full Text

Duke Authors

Cited Authors

  • Lu, M; Wong, F

Published Date

  • December 1987

Published In

Volume / Issue

  • 28 / 12

Start / End Page

  • 2092 - 2095

PubMed ID

  • 3679757

Pubmed Central ID

  • 3679757

International Standard Serial Number (ISSN)

  • 0146-0404

Language

  • eng

Conference Location

  • United States