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Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs.

Publication ,  Journal Article
Peterson, LR; Liesenfeld, O; Woods, CW; Allen, SD; Pombo, D; Patel, PA; Mehta, MS; Nicholson, B; Fuller, D; Onderdonk, A
Published in: J Clin Microbiol
May 2010

The rate of methicillin-resistant Staphylococcus aureus (MRSA) infection continues to rise in many health care settings. Rapid detection of MRSA colonization followed by appropriate isolation can reduce transmission and infection. We compared the performance of the new Roche LightCycler MRSA advanced test to that of the BD GeneOhm MRSA test and culture. Double-headed swabs were used to collect anterior nasal specimens from each subject. For both tests, DNA was extracted and real-time PCR was performed according to manufacturer's instructions. For culture, one swab of the pair was plated directly to CHROMagar MRSA. The swab paired with the BD GeneOhm MRSA test was also placed into an enrichment broth and then plated to CHROMagar MRSA. Colonies resembling staphylococci were confirmed as S. aureus by standard methods. Discrepant specimens had further testing with additional attempts to grow MRSA as well as sample amplicon sequencing. Agreement between results for the two swabs was 99.3% for those with valid results. A total of 1,402 specimens were tested using direct culture detection of MRSA as the gold standard; 187 were culture positive for MRSA. The LightCycler MRSA advanced test had relative sensitivity and specificity of 95.2% (95% confidence interval [CI]: 91.1% to 97.8%) and 96.4% (95% CI: 95.2% to 97.4%), respectively. The BD GeneOhm assay had relative sensitivity and specificity of 95.7% (95% CI: 91.7% to 98.1%) and 91.7% (95% CI: 90.0% to 93.2%), respectively. Following discrepancy analysis, the relative sensitivities of the LightCycler MRSA advanced test and the BD GeneOhm MRSA assay were 92.2 and 93.2%, respectively; relative specificities were 98.9 and 94.2%, respectively. Specificity was significantly better (P<0.001) with the LightCycler MRSA advanced test. The sensitivity of direct culture was 80.4%. The LightCycler MRSA advanced test is a useful tool for sensitive and rapid detection of MRSA nasal colonization.

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Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

May 2010

Volume

48

Issue

5

Start / End Page

1661 / 1666

Location

United States

Related Subject Headings

  • Young Adult
  • Time Factors
  • Staphylococcal Infections
  • Sensitivity and Specificity
  • Polymerase Chain Reaction
  • Nasal Cavity
  • Molecular Diagnostic Techniques
  • Middle Aged
  • Microbiology
  • Methicillin-Resistant Staphylococcus aureus
 

Citation

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Peterson, L. R., Liesenfeld, O., Woods, C. W., Allen, S. D., Pombo, D., Patel, P. A., … Onderdonk, A. (2010). Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. J Clin Microbiol, 48(5), 1661–1666. https://doi.org/10.1128/JCM.00003-10
Peterson, Lance R., Oliver Liesenfeld, Christopher W. Woods, Stephen D. Allen, David Pombo, Parul A. Patel, Maitry S. Mehta, Bradly Nicholson, DeAnna Fuller, and Andrew Onderdonk. “Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs.J Clin Microbiol 48, no. 5 (May 2010): 1661–66. https://doi.org/10.1128/JCM.00003-10.
Peterson, Lance R., et al. “Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs.J Clin Microbiol, vol. 48, no. 5, May 2010, pp. 1661–66. Pubmed, doi:10.1128/JCM.00003-10.
Peterson LR, Liesenfeld O, Woods CW, Allen SD, Pombo D, Patel PA, Mehta MS, Nicholson B, Fuller D, Onderdonk A. Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. J Clin Microbiol. 2010 May;48(5):1661–1666.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

May 2010

Volume

48

Issue

5

Start / End Page

1661 / 1666

Location

United States

Related Subject Headings

  • Young Adult
  • Time Factors
  • Staphylococcal Infections
  • Sensitivity and Specificity
  • Polymerase Chain Reaction
  • Nasal Cavity
  • Molecular Diagnostic Techniques
  • Middle Aged
  • Microbiology
  • Methicillin-Resistant Staphylococcus aureus