CD40 ligand-dependent T cell activation: requirement of B7-CD28 signaling through CD40.


Journal Article

The role of CD40 ligand (CD40L) in the primary activation of T cells is not clear. The cellular and humoral immune responses to adenoviral vectors in a murine model of liver-directed gene transfer were studied to define the mechanisms responsible for CD40L-dependent T cell priming. CD40L-deficient mice did not develop effective cytotoxic T cells to transduced hepatocytes, and T cell-dependent B cell responses were absent. Full reconstitution of cellular and humoral immunity was achieved in CD40L-deficient mice by administration of an activating antibody to CD40 that increased expression of B7.2 on spleen cells. Wild-type mice could be made nonresponsive to vector by administration of antibodies to B7. Thus, CD40L-dependent activation of T cells occurs through signaling of CD40 in the antigen-presenting cell to enhance requisite costimulatory pathways that include B7.

Full Text

Cited Authors

  • Yang, Y; Wilson, JM

Published Date

  • September 1996

Published In

Volume / Issue

  • 273 / 5283

Start / End Page

  • 1862 - 1864

PubMed ID

  • 8791591

Pubmed Central ID

  • 8791591

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.273.5283.1862


  • eng